culture: ender25019
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 30
exposure: 50ms

x-position: 0.03005
y-position: 1.449224
z-position: 43um

positioned over 32, 42, 33, 43 (seemed to have some active units


label is pretty faint again (what's the deal?  maybe culture room is colder on weekends?)
25 is probably okay, but i'll go to 30 so that i can better see calcium signals after TTX is added, and i'll do 3-minute protocols only

the MEA recording itself is amazing.  really nice culture here.


imaging notes: Ender25019_ROI-01_no-drug_1
---------------------
num_frames: 2400
total time: 199.232
corresponding MCD file: Ender25019_ROI-01_no-drug_0001.mcd
lots of nice bursting


ADDED TTX AT 1:45AM
recording for 10min
channel 17 is (as usual) crappy
and 37


imaging notes: Ender25019_ROI-01_TTX_2
---------------------
time: 2:00AM
num_frames: 2400
total time: 199.3454
corresponding MCD file: Ender25019_ROI-01_TTX_0002.mcd
(MEA recording failed at 201s, but that's okay because it's everything i need anyway)


imaging notes: Ender25019_ROI-01_TTX_6
---------------------
time: 2:50AM
num_frames: 2400
total time: 199.219 s
corresponding MCD file: Ender25019_ROI-01_TTX_0006.mcd



imaging notes: Ender25019_ROI-01_TTX_8
---------------------
time: 4:45AM
num_frames: 2400
total time: 199.377 s
corresponding MCD file: Ender25019_ROI-01_TTX_0008.mcd



ADDED CTZ at 4:50AM
recorded for 10min on MEA


imaging notes: Ender25019_ROI-01_TTX-CTZ_2
---------------------
time: 5:05AM
num_frames: 2400
total time: 199.094 s
corresponding MCD file: Ender25019_ROI-01_TTX-CTZ_0002.mcd

next time i do one of these long ones, maybe reduce recording to 1min for post-TTX.


imaging notes: Ender25019_ROI-01_TTX-CTZ_2
---------------------
time: 5:15AM
num_frames: 2400
total time: 199.163 s
corresponding MCD file: Ender25019_ROI-01_TTX-CTZ_0002.mcd