culture: dingo22568 age: 13 DIV temperature: 30C ROI: 01 x-position: 0.62312 y-position: 2.26919 z-position: 44 um positioned over 53, 63, 64, 54 recording for 5min, with 10s before and after test in other ROIs seem to indicate that at least an LED level of 30 is necessary. so i'm gonna go to 35, but both bother to bleach. there's a bunch of good label in this area and there are APs on the MEA in this part of the dish. imaging notes: dingo22568_ROI-04_Rhod-3_no-drug --------------------- num_frames: 4100 imaging notes: dingo22568 total time: 339.8464 s MCD file: Dingo22568_13DIV_no-drug_0009.mcd ADDED TTX AT 7:50PM. RECORDED FOR 10 MIN ON MEA. some false spiking on 17 and 37 like in other recordings. imaging notes: dingo22568_ROI-04_Rhod-3_TTX)1 --------------------- num_frames: 4100 imaging notes: dingo22568 total time: 339.527 s MCD: Dingo22568_13DIV_TTX_0002.mcd ADDED CTZ at 8:10 PM RECORDED FOR 10 MIN ON MEA imaging notes: dingo22568_ROI-04_Rhod-3_TTX-CTZ_1 --------------------- num_frames: 4100 imaging notes: dingo22568 total time: 339.385 s MCD: Dingo22568_13DIV_TTX-CTZ_0002.mcd imaging notes: Dingo22568_ROI-04_Rhod-3_TTX-CTZ_2 --------------------- num_frames: 3650 (early failure) imaging notes: dingo22568 total time: 294.7924 s MCD: Dingo22568_13DIV_TTX-CTZ_0006.mcd