{ "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "FrameKey-0-0-3": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 3, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0c25cb6f-7633-4cb1-b31a-27c1ff1d3edf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 660, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__003.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 30.5 }, "FrameKey-0-0-31": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 31, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9605ecc8-6edc-49df-8e4e-2a9ebe41fda5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3161, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__031.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 31, "ZPositionUm": 44.5 }, "FrameKey-0-0-43": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 43, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "223ad9df-470a-4705-be36-0355a717103d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4233, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__043.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 43, "ZPositionUm": 50.5 }, "FrameKey-0-0-40": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 40, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3c0fd65-2ab3-451d-a371-72caa7dfc905", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3963, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__040.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 40, "ZPositionUm": 49 }, "FrameKey-0-0-14": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 14, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b60a2ea-ab7b-4851-8d83-61bc5818fd0e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1642, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__014.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 36 }, "FrameKey-0-0-55": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 55, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3a08945-21ec-462b-8d14-490a16ae1302", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5302, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__055.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 55, "ZPositionUm": 56.5 }, "FrameKey-0-0-11": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 11, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3687beaa-9801-4463-bd48-64db6e741d61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1373, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__011.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 34.5 }, "FrameKey-0-0-45": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 45, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fa1699a-6b96-43af-870f-04e96cacfe9a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4411, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__045.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 45, "ZPositionUm": 51.5 }, "FrameKey-0-0-37": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 37, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "de5888ed-aa55-4463-82b9-0bc0331698f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3696, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__037.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 37, "ZPositionUm": 47.5 }, "FrameKey-0-0-58": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 58, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7c65e38e-4ba9-4f1b-90bd-d807e356f031", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5666, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__058.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 58, "ZPositionUm": 58 }, "FrameKey-0-0-26": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 26, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1ca90d2e-1ef4-4cb5-8e72-2abafec03a6f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2714, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__026.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 42 }, "FrameKey-0-0-7": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 7, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "829b1cd6-91aa-4a73-bd8c-ee4d1987e3b9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1016, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__007.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 32.5 }, "FrameKey-0-0-8": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 8, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "caac40c1-f076-44fe-8446-a27dd8aa6171", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1105, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__008.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 33 }, "FrameKey-0-0-39": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 39, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "262ff815-0d25-4450-b757-fae14ac2d316", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3874, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__039.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 39, "ZPositionUm": 48.5 }, "FrameKey-0-0-9": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 9, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c4a5e914-240c-4e8a-892d-1ec981e939b4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1194, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__009.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 33.5 }, "FrameKey-0-0-60": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": null, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 60, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6e97d3a2-0bfa-48e3-9edb-a4505a3377d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5844, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__060.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 60, "ZPositionUm": 59 }, "FrameKey-0-0-13": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 13, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bdc3e07f-e360-4290-aeac-cfbbfedeab14", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1553, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__013.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 35.5 }, "FrameKey-0-0-21": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 21, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f98d6473-33cc-498d-a60b-6240e4aded2e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2267, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__021.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 39.5 }, "FrameKey-0-0-53": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 53, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e6873e3-2a7a-4990-91a3-0d81d47082b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5125, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__053.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 53, "ZPositionUm": 55.5 }, "FrameKey-0-0-2": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 2, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6fc563d-91a3-4622-ac7c-088465cfe2c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 571, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__002.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 30 }, "FrameKey-0-0-56": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 56, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c8606a2a-2b5d-41bd-bd60-d22a4b92a03d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5391, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__056.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 56, "ZPositionUm": 57 }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "07d62b45-63fe-4d91-8257-e961786a5576", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 390, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 29 }, "FrameKey-0-0-36": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 36, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cb8b9955-16d4-4b56-95c0-b7503c469984", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3608, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__036.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 36, "ZPositionUm": 47 }, "FrameKey-0-0-42": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 42, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "240e4ea9-73d4-4f90-a6af-6fbe1259ba3f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4143, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__042.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 42, "ZPositionUm": 50 }, "FrameKey-0-0-57": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 57, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2131d03e-fb40-43e7-bb73-81b380d0ec6e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5578, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__057.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 57, "ZPositionUm": 57.5 }, "FrameKey-0-0-24": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 24, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b86903b1-6733-4d64-ad8d-57557c3060fd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2537, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__024.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 41 }, "FrameKey-0-0-18": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 18, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "08590d3a-ed6b-4d04-8ed1-e3988a2b48ac", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1998, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__018.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 38 }, "FrameKey-0-0-44": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 44, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f81a6a00-b9e4-4ad7-b3a2-ca7f65373784", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4322, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__044.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 44, "ZPositionUm": 51 }, "FrameKey-0-0-20": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 20, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8cf6d757-dc08-4e7f-81e0-048131114b98", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2176, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__020.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 39 }, "FrameKey-0-0-12": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 12, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8627aad6-5324-4a5e-a142-bfae8c410f08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1462, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__012.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 35 }, "FrameKey-0-0-15": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 15, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b33ffa19-1503-4ae7-91bd-69cfc9f7fdff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1731, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__015.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 36.5 }, "FrameKey-0-0-22": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 22, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e8d15e6-6aff-48f1-8176-34daa51da266", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2356, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__022.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 40 }, "FrameKey-0-0-1": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 1, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "59d22916-20c2-434a-b521-a1e72b99d054", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 479, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__001.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 29.5 }, "FrameKey-0-0-41": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 41, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ad8fb9d-9dc3-40e0-b83a-66d237f42171", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4052, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__041.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 41, "ZPositionUm": 49.5 }, "FrameKey-0-0-38": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 38, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be75d368-b18c-4c04-a9f1-2985e4424094", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3786, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__038.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 38, "ZPositionUm": 48 }, "FrameKey-0-0-25": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 25, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e5af86c3-ab7b-4060-a060-c4a661c1edb1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2625, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__025.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 41.5 }, "FrameKey-0-0-48": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 48, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "704e9e70-0e5a-491a-a774-33275d5d17c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4679, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__048.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 48, "ZPositionUm": 53 }, "FrameKey-0-0-35": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 35, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ce801534-2f3b-441a-8a4d-355ccb19dfa4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3519, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__035.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 35, "ZPositionUm": 46.5 }, "FrameKey-0-0-46": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 46, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7673633-e593-4959-8127-0297c55d7bd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4501, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__046.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 46, "ZPositionUm": 52 }, "FrameKey-0-0-23": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 23, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e323e511-4ca6-452f-85c5-76c95193bb92", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2446, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__023.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 40.5 }, "FrameKey-0-0-6": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 6, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3a88d5e9-7e3c-4add-860c-d30c0495130e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 927, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__006.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 32 }, "FrameKey-0-0-32": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 32, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a7b37c64-5c5b-4235-91fb-0f6351fca72f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3251, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__032.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 32, "ZPositionUm": 45 }, "FrameKey-0-0-52": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 52, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f8a8c4f4-1634-44b0-b3b3-33c9f8b617a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5034, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__052.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 52, "ZPositionUm": 55 }, "FrameKey-0-0-34": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 34, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42beb89c-6f16-4379-b44c-33a085f86829", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3428, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__034.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 34, "ZPositionUm": 46 }, "FrameKey-0-0-27": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 27, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5e4c3a1f-7adb-4a4d-bc96-7a2ade56d102", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2803, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__027.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 42.5 }, "FrameKey-0-0-50": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 50, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3e49031-231d-442a-89f5-bd4e3cf5c43a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4858, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__050.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 50, "ZPositionUm": 54 }, "FrameKey-0-0-5": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 5, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e67a555-0c1f-48e8-874c-71a27fd0834e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 838, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__005.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 31.5 }, "FrameKey-0-0-59": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 59, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9d94d10a-b597-40e1-87dc-540426cde6fb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5755, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__059.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 59, "ZPositionUm": 58.5 }, "FrameKey-0-0-19": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 19, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9af8979f-de2c-4a60-b0ee-14d19fe0462d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2086, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__019.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 38.5 }, "FrameKey-0-0-28": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 28, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9091df1-b03a-4f3a-b670-522b1aad4a8e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2892, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__028.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 43 }, "FrameKey-0-0-49": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 49, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57d4609d-08da-4822-bbb0-53d857020ecf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4770, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__049.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 49, "ZPositionUm": 53.5 }, "FrameKey-0-0-47": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 47, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "855a0e38-e719-4ed9-9cf3-f79a82155fc2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4590, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__047.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 47, "ZPositionUm": 52.5 }, "FrameKey-0-0-51": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:24 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 51, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "453e9d6c-3509-4a80-a2b4-659576c5d080", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4946, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__051.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 51, "ZPositionUm": 54.5 }, "FrameKey-0-0-33": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:23 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 33, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1de2f7a0-4113-42ed-b64b-6a0ddd57a2d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3340, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__033.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 33, "ZPositionUm": 45.5 }, "FrameKey-0-0-29": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 29, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e87079e3-043d-4fb6-b8e0-316229020af6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2981, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__029.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 43.5 }, "FrameKey-0-0-54": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:25 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 54, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "cfb62c1e-2232-42c4-acfd-204c9b076d06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5213, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__054.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 54, "ZPositionUm": 56 }, "FrameKey-0-0-17": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 17, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "608ec257-6a79-4849-a48f-690a51678575", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1909, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__017.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 37.5 }, "FrameKey-0-0-4": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:20 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 4, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a7f25f6-4d69-445d-9acc-1c982b01c563", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 749, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__004.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 31 }, "FrameKey-0-0-10": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 10, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9dcdbea2-b4eb-43fe-9d53-c099f3826986", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1284, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__010.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 34 }, "FrameKey-0-0-30": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:22 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 30, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f427d4ef-3874-4824-8368-78132e81e384", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3070, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__030.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 44 }, "FrameKey-0-0-16": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "-12", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-18 19:29:21 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 16, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aac1a503-956a-45aa-b7bc-75ceba051c08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1820, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "a46d5d41-807d-48f9-839f-6185b71fab62", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-18 19:29:20 -0400", "Date": "2014-07-18", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Dingo22568_ROI-04_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__016.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 37 } }