culture: coolio25024
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 20 (unless otherwise specified in filename)
blue LED level: off
exposure: 50ms

x-position: 0.10450
y-position: 0.89467
z-position: 29.5um

electrode 44 was super active, and the other nearby active electrodes were 33 and 34.  so i positioned objective over 33, 34, 43, and 44.  the activity and bursting is occurring across the array, so that is fantastic.

recording for 5min, with 10s before and after


there's something wrong with channel 37 and noise.  excluse that one from today's analysis.



imaging notes: coolio25022_ROI-01_quick-test_exp-50ms_LED-20
---------------------
num_frames: 350
total time: 30.4702 s
corresponding MCD file: none


imaging notes: coolio25024_ROI-01_no-drug_2
---------------------
num_frames: 4100
total time:  339.4054 s
corresponding MCD file: coolio25024_ROI-01_no-drug_0002.mcd
lots of bursting.  this should be a good one.  i took a screenshot of MCRack during recording


ADDED 1uM TTX AT 7:00PM
electrode 17 is again active, which is starting to make me suspicious of whether this is a real AP or just noise on the channel.


imaging notes: coolio25024_ROI-01_TTX_1
---------------------
num_frames: 4100
total time:  339.6004 s
corresponding MCD file: coolio25024_ROI-01_TTX_0002.mcd (NOTE: accidentally left this as the 630s recording, so will need to stop manually)

channel 17 and 37 are again the annoying ones.  i'm starting to believe these aren't real.  will put TTX at end of my next prep to see again.

ADDED 1uM CTZ AT 7:24PM
recorded for 10min 30s on MEA



imaging notes: coolio25024_ROI-01_TTX-CTZ_1
---------------------
num_frames: 4100
total time:  339.432 s
corresponding MCD file: coolio25024_13DIV_TTX-CTZ_0004.mcd

what's really cool is you can definitely see the glia blinking...!


since i have time, before my next culture, i'll do a 10-minute recording here (i can use this to assess bleaching at LED=20)


imaging notes: coolio25024_ROI-01_TTX-CTZ_1
---------------------
num_frames: 8100
total time:  610.6798 s (errored out, but it's okay; this is plenty)
corresponding MCD file: coolio25024_13DIV_TTX-CTZ_0009.mcd (errored out at 615s, but that is enough)