culture: coolio25022
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 30 (unless otherwise specified in filename)
blue LED level: off
exposure: 50ms

x-position: -0.20820
y-position: 1.12358
z-position: 26um

because electrode 23 was quite active, and 34 had a nice unit, so I positioned objective over electrodes 23, 24, 33, and 34


recording for 5min, with 10s before and after

overall this label was worse, so had to use higher light level.  hopefully won't bleach too much.

also, the activity wasn't as impressive on the MEA recording overall.


imaging notes: coolio25022_ROI-01_quick-test_exp-50ms_LED-20
---------------------
num_frames: 750
total time: 65.1628 s
corresponding MCD file: none


imaging notes: coolio25022_ROI-01_quick-test_exp-50ms_LED-25
---------------------
num_frames: 750
total time: 65.1501 s
corresponding MCD file: none


imaging notes: coolio25022_ROI-01_quick-test_exp-50ms_LED-30
---------------------
num_frames: 750
total time: 65.1638 s
corresponding MCD file: none


imaging notes: coolio25022_ROI-01_no-drug_2
---------------------
num_frames: 4100
total time:  339.1804 s
corresponding MCD file: coolio25022_ROI-01_no-drug_0004.mcd


****** ADDED TTX AT 5:10PM ******
recorded for 10min
a couple spikes near back of culture (not well-mixed?)
re-mixing and letting it go another 5min
electrode 17 still going for it.  maybe TTX-insensitive?


imaging notes: coolio25022_ROI-01_TTX_2
---------------------
num_frames: 4100
total time:  339.349 s
corresponding MCD file: coolio25022_ROI-01_TTX_0002.mcd


****** ADDED CTZ AT 5:45PM ******
recorded for 10min, but RAM ran out at ~6min in
a couple spikes near back of culture (not well-mixed?)

i'll record another 5min or so
electrode 17 is still active.

electrode 37 was periodically have noise on it before, so watch out for that in analysis, which seems to be periodically an issue.  consider excluding this channel from analysis.


imaging notes: coolio25022_ROI-01_TTX-CTZ_2
---------------------
num_frames: 4100
total time:  ?? s
corresponding MCD file: coolio25022_ROI-01_TTX-CTZ_0002
error partway through... (circular buffer overflow)
only got to 2635 frames...

redo...


imaging notes: coolio25022_ROI-01_TTX-CTZ_4
---------------------
num_frames: 4100
total time: 339.592s
corresponding MCD file: coolio25022_ROI-01_TTX-CTZ_0004.mcd