culture: finn24595
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 20
blue LED level: 75
exposure: 50ms

x-position: 0.02238
y-position: 1.40263
z-position: 47um

bursts are happening about once every minute or two, and only on the bottom half of the array

i moved around the array a lot and had a hard time finding a spot that would evoke bursts (in no drug) with blue light stim.  I finally found a spot, but it was somewhat off the array.

unfortunately 17 and 34 are bad again (too bad b/c they have some nice real units)


doing 3-minute protocols
(camera on at start, imaging light on at 10s, blue light on at 40s and 160s, imaging light off at 190s)



imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_no-drug_ChR2-stim_1
---------------------
num_frames:2400
time: 198.977s
MCD: Finn24595_10DIV_ROI-03_no-drug_ChR2-stim_0002.mcd

first stimulus did nothing (burst ~20s beforehand)
second stimulus evoked a great burst


same but with light off


imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_no-drug_no-stim_1
---------------------
num_frames: 2400
time: 199.0804
MCD: none

bursts around 80s and 130s

ugh!  MCS recording didn't work.  will try again...



imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_no-drug_no-stim_2
---------------------
num_frames: 2400
time: 199.142
MCD: Finn24595_10DIV_ROI-03_no-drug_no-stim_0003.mcd

early bursts.  good.



ADDED CNQX AT 8:30PM


imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_CNQX_no-stim_1
---------------------
num_frames: 2400
time: 199.529s
MCD: Finn24595_10DIV_ROI-03_CNQX_no-stim_0002.mcd


now turned back on blue light


imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_CNQX_ChR2-stim_1
---------------------
num_frames: 2400
time: 198.5774
MCD: Finn24595_10DIV_ROI-03_CNQX_ChR2-stim_0002.mcd

stimuli barely evoked anything
it could be that these cultures are just too young / poorly connected
or could be minor infection (like the others)



imaging notes: Finn24595_10DIV_ROI-01_Rhod-3_CNQX_ChR2-stim_2
---------------------
num_frames: 2400
time: 199.7304 s
MCD: Finn24595_10DIV_ROI-03_CNQX_ChR2-stim_0006.mcd

again barely anything