{ "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "FrameKey-0-0-3": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 3, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "63c38eff-aab0-46bf-9a14-46748f229771", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 962, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__003.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 40.5 }, "FrameKey-0-0-31": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 31, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "532d525b-e635-4050-b1a3-fbf25dda96ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5711, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__031.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 31, "ZPositionUm": 54.5 }, "FrameKey-0-0-43": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 43, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb3c3915-9f50-4671-bece-7ed6e2474ebe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7750, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__043.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 43, "ZPositionUm": 60.5 }, "FrameKey-0-0-40": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 40, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d53bf617-e9fd-4a0e-999a-28098d30cde6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7239, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__040.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 40, "ZPositionUm": 59 }, "FrameKey-0-0-14": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 14, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1a860334-ca4b-405c-9e0e-e649bd85f1cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2830, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__014.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 46 }, "FrameKey-0-0-55": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 55, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1df6145a-eefb-47e5-b087-c9d4c357744d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9790, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__055.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 55, "ZPositionUm": 66.5 }, "FrameKey-0-0-11": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 11, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e6c18c68-7dcf-40ec-8f92-48441741431b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2322, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__011.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 44.5 }, "FrameKey-0-0-45": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 45, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5bb5b72c-a3a5-44f2-b1da-f239e843d1e5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8088, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__045.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 45, "ZPositionUm": 61.5 }, "FrameKey-0-0-37": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 37, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b8deaab0-3869-4cc4-8c36-468a53e3d6f4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6732, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__037.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 37, "ZPositionUm": 57.5 }, "FrameKey-0-0-58": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 58, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d05b3352-41b2-425a-8c34-4ffeb77c0a46", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10297, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__058.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 58, "ZPositionUm": 68 }, "FrameKey-0-0-26": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 26, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48e4114a-d15d-4213-a132-c996bddc6ceb", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4862, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__026.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 52 }, "FrameKey-0-0-7": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 7, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ee76c573-fcb6-4507-b503-a44a970bc3de", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1642, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__007.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 42.5 }, "FrameKey-0-0-8": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 8, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6f01d951-f890-48f6-aa6c-5cb47c343a08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1811, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__008.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 43 }, "FrameKey-0-0-39": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 39, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ebbc82dc-4f74-4c2f-81f6-6c5a0ac9d57d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7070, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__039.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 39, "ZPositionUm": 58.5 }, "FrameKey-0-0-9": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 9, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb9992d0-1f43-4bf9-bcb3-8dc512b26379", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1980, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__009.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 43.5 }, "FrameKey-0-0-60": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": null, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 60, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "54fde2cb-cbbe-4964-a4fb-79d370a15b28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10638, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__060.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 60, "ZPositionUm": 69 }, "FrameKey-0-0-13": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 13, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4d19d772-971f-4887-89b8-8748768a5d15", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2660, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__013.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 45.5 }, "FrameKey-0-0-21": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 21, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fad7edf-b818-4439-b775-b7ead4b31d61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4016, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__021.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 49.5 }, "FrameKey-0-0-53": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 53, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa36be1e-f747-462b-9383-aabb3e9a8621", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9448, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__053.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 53, "ZPositionUm": 65.5 }, "FrameKey-0-0-2": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 2, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da5bd0d1-6aba-4085-a07c-bebbeb60dc04", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 792, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__002.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 40 }, "FrameKey-0-0-56": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 56, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "93b464bf-886d-4320-b91f-d566739b93f3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9959, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__056.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 56, "ZPositionUm": 67 }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f39423d8-9d8a-4078-a2d8-6c2b75e8545f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 451, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 39 }, "FrameKey-0-0-36": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 36, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f4b959db-6d88-48fa-80f2-b1eecb7ccbb5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6560, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__036.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 36, "ZPositionUm": 57 }, "FrameKey-0-0-42": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 42, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "27a195e0-03f4-4e80-b07b-f54f431338c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7581, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__042.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 42, "ZPositionUm": 60 }, "FrameKey-0-0-57": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 57, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7da143a5-1bd5-4a5c-b536-922795982f33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10128, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__057.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 57, "ZPositionUm": 67.5 }, "FrameKey-0-0-24": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 24, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f3ec584-d192-4241-98d3-85271008d86e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4523, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__024.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 51 }, "FrameKey-0-0-18": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 18, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a9b04e39-c5d1-4ef3-96b5-6fb21f686f3a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3509, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__018.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 48 }, "FrameKey-0-0-44": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 44, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a08cea07-d4ad-4686-aa65-93932b9be2ce", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7919, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__044.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 44, "ZPositionUm": 61 }, "FrameKey-0-0-20": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 20, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "003a2e82-511e-43f0-9a57-298e43ac73a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3847, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__020.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 49 }, "FrameKey-0-0-12": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 12, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b2b5147-7261-4dca-9311-e4b33d831b51", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2491, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__012.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 45 }, "FrameKey-0-0-15": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 15, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2f64c294-7e61-4156-b70b-c4c1a2c6504b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2999, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__015.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 46.5 }, "FrameKey-0-0-22": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 22, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "afd13097-485a-4cd3-8f10-6e2387e8c706", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4186, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__022.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 50 }, "FrameKey-0-0-1": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:31 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 1, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2be00ed7-443d-4ba9-a8e6-ec773621b734", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 620, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__001.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 39.5 }, "FrameKey-0-0-41": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 41, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "43e4a7d1-368b-4b03-b8aa-fa67059962e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7408, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__041.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 41, "ZPositionUm": 59.5 }, "FrameKey-0-0-38": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:38 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 38, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4045e23e-1d7c-4702-a23c-0797ee52dd23", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6901, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__038.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 38, "ZPositionUm": 58 }, "FrameKey-0-0-25": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 25, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b4180d4b-d4b3-4aa9-ba0b-e2281461709a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4692, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__025.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 51.5 }, "FrameKey-0-0-48": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 48, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "19cdf830-edfb-4c28-b3a7-dbc1400ada2c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8599, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__048.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 48, "ZPositionUm": 63 }, "FrameKey-0-0-35": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 35, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "42319e41-0acb-44de-b458-484823779b74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6391, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__035.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 35, "ZPositionUm": 56.5 }, "FrameKey-0-0-46": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 46, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e232c823-5bbe-4819-8d14-b315209289c1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8260, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__046.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 46, "ZPositionUm": 62 }, "FrameKey-0-0-23": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:35 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 23, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c9d0f07c-0388-4a42-9913-ca4aefec311f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4355, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__023.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 50.5 }, "FrameKey-0-0-6": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 6, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ed8309d5-3078-4cf7-a527-d069055700a3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1470, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__006.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 42 }, "FrameKey-0-0-32": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 32, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "da0ad4fb-a6f8-4d11-9fcc-9202abc0076d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5880, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__032.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 32, "ZPositionUm": 55 }, "FrameKey-0-0-52": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 52, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7edb7d7f-e944-4abf-98de-4a3e70511715", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9279, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__052.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 52, "ZPositionUm": 65 }, "FrameKey-0-0-34": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 34, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f15f6087-f500-441d-a05a-3ad0c5c3c20f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6222, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__034.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 34, "ZPositionUm": 56 }, "FrameKey-0-0-27": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 27, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a1246d94-0c5d-4416-acd0-1d4cc46d8a4e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5031, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__027.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 52.5 }, "FrameKey-0-0-50": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 50, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "170200c5-6a8e-4720-8543-a5ac417568e2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8938, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__050.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 50, "ZPositionUm": 64 }, "FrameKey-0-0-5": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 5, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7f6bcd7d-dfbe-4b61-9ed6-2327aa1e28a9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1300, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__005.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 41.5 }, "FrameKey-0-0-59": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:41 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 59, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "89724964-a775-476d-a90e-74009260da34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10469, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__059.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 59, "ZPositionUm": 68.5 }, "FrameKey-0-0-19": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 19, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5eb57696-373f-4918-9c4a-ae839f7c8d31", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3678, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__019.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 48.5 }, "FrameKey-0-0-28": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 28, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d090b360-7bb7-4336-a96b-35e6b6420d33", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5200, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__028.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 53 }, "FrameKey-0-0-49": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 49, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6379c031-76ea-4358-9936-87a5a5ac1798", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8768, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__049.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 49, "ZPositionUm": 63.5 }, "FrameKey-0-0-47": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:39 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 47, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "11e2befa-b1a3-4dd2-8646-6c97b82ab26b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8430, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__047.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 47, "ZPositionUm": 62.5 }, "FrameKey-0-0-51": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 51, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c2ffbb13-3111-4daa-8870-e29a72e62a63", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9109, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__051.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 51, "ZPositionUm": 64.5 }, "FrameKey-0-0-33": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:37 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 33, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a26c2dc3-08ac-4399-b95b-0ba3dbdc297c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6052, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__033.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 33, "ZPositionUm": 55.5 }, "FrameKey-0-0-29": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 29, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7219c29e-7c39-4a98-94ef-427a7028ad08", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5372, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__029.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 53.5 }, "FrameKey-0-0-54": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:40 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 54, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1eb4ff16-9505-47f2-87f1-550e7f9b1242", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9617, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__054.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 54, "ZPositionUm": 66 }, "FrameKey-0-0-17": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 17, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "48a6e35f-2c28-44a2-9fd8-cea92510a69d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3339, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__017.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 47.5 }, "FrameKey-0-0-4": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:32 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 4, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1d75ebcc-167c-4100-bf2b-d8f3e0a2bf4a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1131, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__004.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 41 }, "FrameKey-0-0-10": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:33 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 10, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74d67f98-362c-4759-aa79-8545588507c9", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2150, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__010.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 44 }, "FrameKey-0-0-30": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:36 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 30, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "74dc212c-1dd8-4c07-a188-9fee62ee0256", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5542, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__030.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 54 }, "FrameKey-0-0-16": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "30", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 20:03:34 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 16, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d59fdb1-426e-4f33-87d9-664e8fa542a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3170, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "ca75a78c-e259-4ac0-8b41-41bb675cb3c3", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 20:03:32 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-03_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__016.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 47 } }