{ "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Finn24595_10DIV_ROI-01_eYFP_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "FrameKey-0-0-3": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 3, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9f747cf4-d555-4cea-a21b-da2e0401b877", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 965, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__003.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 34.5 }, "FrameKey-0-0-31": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 31, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a600d9b-90cc-4d1b-bba9-320f2a0fe9c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5717, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__031.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 31, "ZPositionUm": 48.5 }, "FrameKey-0-0-43": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 43, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c7e6ec3-b0ea-4f80-9e8a-4f7ad1e922c3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7754, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__043.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 43, "ZPositionUm": 54.5 }, "FrameKey-0-0-40": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 40, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1052efd1-1af6-421a-9470-85f9cdfc1605", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7244, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__040.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 40, "ZPositionUm": 53 }, "FrameKey-0-0-14": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 14, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f12e5fe6-6c87-469a-ac2b-e2fc3e449030", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2833, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__014.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 40 }, "FrameKey-0-0-55": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 55, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d522085e-5d08-4a97-8d3d-9ce5da795e2b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9789, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__055.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 55, "ZPositionUm": 60.5 }, "FrameKey-0-0-11": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 11, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f49e8f51-43ea-4100-acb2-0fb2d310676c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2323, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__011.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 38.5 }, "FrameKey-0-0-45": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 45, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1fa0bb02-c671-43d1-b1cd-cb16a202619b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8092, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__045.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 45, "ZPositionUm": 55.5 }, "FrameKey-0-0-37": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 37, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d94248c-2fb4-4449-933e-75c1d985ffc1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6736, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__037.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 37, "ZPositionUm": 51.5 }, "FrameKey-0-0-58": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 58, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eadc919d-2026-48db-9c9f-6e181a94e48a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10298, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__058.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 58, "ZPositionUm": 62 }, "FrameKey-0-0-26": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 26, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2ed48b27-9ed4-4613-9447-13a1b24c9a5d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4869, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__026.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 46 }, "FrameKey-0-0-7": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 7, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6046163-2741-40b6-b28c-d02551f881e7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1644, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__007.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 36.5 }, "FrameKey-0-0-8": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 8, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2c3e9807-1ae3-47f1-9b44-60379daf103c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1814, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__008.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 37 }, "FrameKey-0-0-39": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 39, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8892eece-ab84-4a12-af9c-c16468ff8469", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7075, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__039.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 39, "ZPositionUm": 52.5 }, "FrameKey-0-0-9": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 9, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6bbc98cd-28f5-4e25-ab95-73fa3f0abb6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1984, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__009.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 37.5 }, "FrameKey-0-0-60": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": null, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 60, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "645c1aa7-e73a-4c43-a7a0-9d1f9987ffee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10638, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__060.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 60, "ZPositionUm": 63 }, "FrameKey-0-0-13": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 13, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "49e996a2-1998-442e-a1dd-c975c1a249a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2664, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__013.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 39.5 }, "FrameKey-0-0-21": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 21, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d127854f-7a30-473e-a1e8-3c0f0e35a120", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4020, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__021.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 43.5 }, "FrameKey-0-0-53": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 53, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3192b503-0869-4ff7-b63a-aa31821278fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9450, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__053.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 53, "ZPositionUm": 59.5 }, "FrameKey-0-0-2": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 2, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "425d1d06-2f4e-4dc6-bf60-be784613a5ee", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 796, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__002.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 34 }, "FrameKey-0-0-56": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 56, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f3f08465-8c4a-4927-96a1-bfdd7d6ec201", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9958, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__056.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 56, "ZPositionUm": 61 }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a15e0071-0ab8-421e-b71e-71195e9ce1b6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 456, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 33 }, "FrameKey-0-0-36": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 36, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6b4d711c-9704-448d-b71c-cd58fb9cb5d5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6564, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__036.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 36, "ZPositionUm": 51 }, "FrameKey-0-0-42": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 42, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "47e5e8cb-c88c-4f37-bad4-a203bb82a703", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7585, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__042.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 42, "ZPositionUm": 54 }, "FrameKey-0-0-57": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 57, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60702f94-9467-450a-8358-9b5e8c5e71ab", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10127, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__057.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 57, "ZPositionUm": 61.5 }, "FrameKey-0-0-24": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 24, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1b10a291-815a-4a19-af78-9b24f63399ff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4528, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__024.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 45 }, "FrameKey-0-0-18": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 18, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0e0c8ecd-7a53-46e5-a0e3-466d613a950b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3510, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__018.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 42 }, "FrameKey-0-0-44": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 44, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a945a198-0e0a-44b5-90fa-7e914f44f2c4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7923, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__044.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 44, "ZPositionUm": 55 }, "FrameKey-0-0-20": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 20, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ae8a8f8e-c62c-449e-b91d-59fe02c29b94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3849, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__020.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 43 }, "FrameKey-0-0-12": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 12, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "31d21420-b05f-4f6a-9e97-69d07f43d9e8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2494, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__012.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 39 }, "FrameKey-0-0-15": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 15, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aaf1dc07-6014-4959-a91f-c868ba58860d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3002, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__015.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 40.5 }, "FrameKey-0-0-22": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 22, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c68b7f0d-b60f-4457-bdf6-a9c791fedc79", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4189, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__022.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 44 }, "FrameKey-0-0-1": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 1, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3875fcb-4490-49be-a30b-1d1f19fd909b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 626, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__001.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 33.5 }, "FrameKey-0-0-41": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 41, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0de9936b-a629-4670-8311-11f970841866", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7413, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__041.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 41, "ZPositionUm": 53.5 }, "FrameKey-0-0-38": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 38, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5099c22d-d41b-46da-8992-b834a3347b07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6906, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__038.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 38, "ZPositionUm": 52 }, "FrameKey-0-0-25": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 25, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3b41e99c-0634-4d16-a282-c980d9c3491d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4698, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__025.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 45.5 }, "FrameKey-0-0-48": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 48, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "792500ba-1ba8-479d-b16e-be1bfd098ca2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8602, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__048.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 48, "ZPositionUm": 57 }, "FrameKey-0-0-35": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 35, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a11e2b7-e4b9-4c0c-aa77-635205b21f76", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6394, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__035.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 35, "ZPositionUm": 50.5 }, "FrameKey-0-0-46": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 46, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "94d0e86c-4f61-45a1-9a1b-4c9ddd33f24d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8262, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__046.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 46, "ZPositionUm": 56 }, "FrameKey-0-0-23": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 23, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "009763d9-d33b-4f13-a222-4a61e7d4c37a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4359, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__023.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 44.5 }, "FrameKey-0-0-6": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 6, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e8985a6-e660-47b5-8956-f0196771f012", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1473, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__006.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 36 }, "FrameKey-0-0-32": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 32, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f85aa385-527f-48ac-9987-d1d49db15041", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5886, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__032.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 32, "ZPositionUm": 49 }, "FrameKey-0-0-52": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 52, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b46408b-1f8e-4407-9039-bee17ecb5c07", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9279, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__052.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 52, "ZPositionUm": 59 }, "FrameKey-0-0-34": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 34, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "817bc998-ac2c-404c-a562-0ff9111f29e1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6225, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__034.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 34, "ZPositionUm": 50 }, "FrameKey-0-0-27": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 27, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "693d90db-0636-4073-a1c5-ebee00393627", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5038, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__027.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 46.5 }, "FrameKey-0-0-50": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 50, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b7347014-3f3b-4c2d-8deb-fbc8c7151e06", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8941, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__050.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 50, "ZPositionUm": 58 }, "FrameKey-0-0-5": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 5, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d762c84-1d55-41e2-9d1b-ae8dafda69ed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1304, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__005.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 35.5 }, "FrameKey-0-0-59": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 59, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d2360b90-4530-456d-a648-6f30f8fe3cda", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 10467, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__059.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 59, "ZPositionUm": 62.5 }, "FrameKey-0-0-19": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 19, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d575674f-567f-48b8-b28e-b013d87dcac4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3680, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__019.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 42.5 }, "FrameKey-0-0-28": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 28, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c6700d96-e02c-42d0-91a7-3fe7902e754b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5207, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__028.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 47 }, "FrameKey-0-0-49": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 49, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9e2dd606-925e-46f5-a7ba-2660c3a9ead7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8771, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__049.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 49, "ZPositionUm": 57.5 }, "FrameKey-0-0-47": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 47, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28e93cbb-da9d-4941-bb70-80851ffd714c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 8431, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__047.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 47, "ZPositionUm": 56.5 }, "FrameKey-0-0-51": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 51, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2865c054-5e55-4faf-940e-e8c5b35c9356", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9110, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__051.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 51, "ZPositionUm": 58.5 }, "FrameKey-0-0-33": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 33, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6db3489f-6245-4aff-86e0-74fd79ce1b1f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6056, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__033.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 33, "ZPositionUm": 49.5 }, "FrameKey-0-0-29": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 29, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "70fd40f2-3ac9-4d5d-918a-1006688d934f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5377, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__029.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 47.5 }, "FrameKey-0-0-54": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 54, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5d38819b-0aee-4b46-92fb-3eb425ef3b3d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 9620, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__054.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 54, "ZPositionUm": 60 }, "FrameKey-0-0-17": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:49 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 17, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a42d96de-d683-4d3c-b60a-171b881185a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3341, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__017.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 41.5 }, "FrameKey-0-0-4": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:46 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 4, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3567caaa-0708-4784-b7a5-9876f464ba82", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1134, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__004.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 35 }, "FrameKey-0-0-10": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:47 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 10, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ee38095-7f34-4ad4-ba70-f576682ddc18", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2153, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__010.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 38 }, "FrameKey-0-0-30": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 30, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "00aadded-1387-451e-a0a6-18f70478e4a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5546, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__030.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 48 }, "FrameKey-0-0-16": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "FrameIndex": 0, "COM7-Handshaking": "Off", "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "Camera": "", "quantEM-OutputTriggerFirstMissing": "0", "PIZStage-StepSizeUm": "0.0100", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 100, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-PreampDelay": "1", "quantEM-PrescanPixels": "28", "quantEM-Name": "PM1394Cam00", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "PIZStage-Interface": "Remote: Interface commands mode", "quantEM-ShutterCloseDelay": "0", "Time": "2014-07-22 19:19:48 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "COM7-Verbose": "1", "quantEM-PVCAM Version": "2.7.7", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 16, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "266c03ee-dae4-4302-b551-eb8eb13c30fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3171, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "4a257769-a287-441f-81f5-9161a3a1fa45", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 19:19:46 -0400", "Date": "2014-07-22", "MetadataVersion": 10, "z-step_um": 0.5, "ChContrastMin": [2147483647], "SlicesFirst": true, "PositionIndex": 0, "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Frames": 1, "Prefix": "EnderI5407_13DIV_ROI-01_Rhod-3_CNQX_ChR2-stim", "PixelSize_um": 0, "KeepShutterOpenChannels": false, "BitDepth": 16, "ComputerName": "falcon-cal-imag", "Channels": 1, "Source": "Micro-Manager", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "TimeFirst": false, "ChColors": [-1], "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "J:\\Documents and Settings\\mkuykendal\\My Documents\\mingfaifong_CaImaging\\2014-07-22_EnderI5407_MEA_Rhod-3_CNQX-ChR2-stim" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "100.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__016.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 41 } }