culture: enderI5407 age: 13 DIV temperature: 30C ROI: 01 white LED level: 20 blue LED level: 75 exposure: 50ms x-position: -0.52769 y-position: 1.45814 z-position: 48umum MEA is bad. just doing the imaging recording. doing 3-minute protocols (camera on at start, imaging light on at 10s, blue light on at 40s and 160s, imaging light off at 190s) imaging notes: EnderI5407_ROI-01_Rhod-3_no-drug_ChR2-stim_1 --------------------- num_frames: 2400 total time: 199.528 s culture is really nice and bursty there is clearly a darker frame between the stim pulse and the evoked burst turned blue light off and repeated protocol... imaging notes: EnderI5407_ROI-01_Rhod-3_no-drug_no-stim_1 --------------------- num_frames: 2400 total time: 199.255 s ADDED CNQX AT 5:12PM since MEA is bad, i'll just wait 10min. imaging notes: EnderI5407_ROI-01_Rhod-3_CNQX_no-stim_1 --------------------- num_frames: 2400 total time: 198.947s turned blue light back on (ready to trigger) imaging notes: EnderI5407_ROI-01_Rhod-3_CNQX_ChR2-stim_1 --------------------- num_frames: 2400 total time: 199.0534s imaging notes: EnderI5407_ROI-01_Rhod-3_CNQX_ChR2-stim_2 --------------------- num_frames: 2400 total time: 198.9164 s imaging notes: EnderI5407_ROI-01_Rhod-3_CNQX_ChR2-stim_3 --------------------- num_frames: 2400 total time: 198.823s