{ "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "FrameKey-0-0-3": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 3, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a5a4d16-0f6d-479e-9cd3-710f120ee322", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 480, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__003.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 36.5 }, "FrameKey-0-0-31": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 31, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f247f488-2cda-4bcc-8042-eb47cf914325", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3817, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__031.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 31, "ZPositionUm": 50.5 }, "FrameKey-0-0-43": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 43, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d717290c-4090-4714-bdea-35330cb5173e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5248, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__043.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 43, "ZPositionUm": 56.5 }, "FrameKey-0-0-40": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 40, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "58619b62-cbd2-4f1f-97f1-2dd79c22c7a2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4893, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__040.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 40, "ZPositionUm": 55 }, "FrameKey-0-0-14": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 14, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "72bd4a7a-6cbb-4a9b-9a74-fb76690a5864", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1790, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__014.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 42 }, "FrameKey-0-0-55": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 55, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3ff16c8c-3897-4b1c-be52-d1eb2998a81c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6677, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__055.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 55, "ZPositionUm": 62.5 }, "FrameKey-0-0-11": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 11, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a03a4cfc-91e4-4225-97d2-40fee27426a8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1435, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__011.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 40.5 }, "FrameKey-0-0-45": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 45, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "651ab138-5342-4e36-a0a0-e5527fd468a7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5486, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__045.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 45, "ZPositionUm": 57.5 }, "FrameKey-0-0-37": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 37, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b795be29-d318-47a5-8304-c2dd86eee608", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4531, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__037.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 37, "ZPositionUm": 53.5 }, "FrameKey-0-0-58": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 58, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "748bc17c-86a2-4d5e-80e9-1fc1e4adc301", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7033, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__058.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 58, "ZPositionUm": 64 }, "FrameKey-0-0-26": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 26, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aa136449-764a-4ecc-b56a-e44d898cf7a5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3224, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__026.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 48 }, "FrameKey-0-0-7": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 7, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bc8c0e96-6f6c-4b27-8431-05f24a032d94", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 960, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__007.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 38.5 }, "FrameKey-0-0-8": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 8, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "412f55e4-b75d-40ff-8f93-8b5f8b057d95", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1079, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__008.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 39 }, "FrameKey-0-0-39": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 39, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ca262280-c25e-4b0d-8c05-5bcfb9930a93", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4767, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__039.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 39, "ZPositionUm": 54.5 }, "FrameKey-0-0-9": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 9, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "87f29c96-af46-474c-b9c9-1ade7809d0f6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1197, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__009.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 39.5 }, "FrameKey-0-0-60": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": null, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 60, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a636b70b-fd0c-4c32-baf9-464319389577", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7271, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__060.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 60, "ZPositionUm": 65 }, "FrameKey-0-0-13": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 13, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "50e0898b-9620-40e5-bb48-dc150042e9ae", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1672, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__013.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 41.5 }, "FrameKey-0-0-21": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 21, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e0817207-70a1-401c-a5eb-a207162525cd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2625, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__021.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 45.5 }, "FrameKey-0-0-53": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 53, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5ea91048-c0b4-4fd3-874a-a14b008f44c6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6436, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__053.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 53, "ZPositionUm": 61.5 }, "FrameKey-0-0-2": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 2, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "275cb276-8361-4aec-aa04-94e361d47fed", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 362, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__002.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 36 }, "FrameKey-0-0-56": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 56, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "56fd0707-abe2-45c4-abd3-bedd8f9c4f7a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6797, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__056.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 56, "ZPositionUm": 63 }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:50 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e57353a2-f825-4856-8e61-b542e616f086", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 124, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 35 }, "FrameKey-0-0-36": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 36, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18447cbf-2677-4cd7-a89b-05690069e953", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4412, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__036.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 36, "ZPositionUm": 53 }, "FrameKey-0-0-42": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 42, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "596c392d-d691-4ada-a1b5-749002108678", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5129, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__042.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 42, "ZPositionUm": 56 }, "FrameKey-0-0-57": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 57, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "75ee599e-2c65-4408-aebb-f1589f44d38d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6915, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__057.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 57, "ZPositionUm": 63.5 }, "FrameKey-0-0-24": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 24, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fb84e00-5195-4e51-8344-68b1a64b73af", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2980, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__024.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 47 }, "FrameKey-0-0-18": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 18, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8c2a91e9-7b60-4492-ae95-120a4caaf5d0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2265, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__018.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 44 }, "FrameKey-0-0-44": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 44, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8beedbbd-d536-4ca2-9427-c4d33e2d79c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5366, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__044.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 44, "ZPositionUm": 57 }, "FrameKey-0-0-20": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 20, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "088ed7b0-fcb6-4745-8497-c90343a13ab8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2506, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__020.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 45 }, "FrameKey-0-0-12": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 12, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c6de04d-2fc8-4140-9e74-34ea794bc33b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1554, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__012.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 41 }, "FrameKey-0-0-15": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 15, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4f74a0ff-70a3-4482-8d43-494cb72b3c60", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1910, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__015.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 42.5 }, "FrameKey-0-0-22": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 22, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0edffa6d-b71d-44ef-8682-2e6df3dd421e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2743, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__022.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 46 }, "FrameKey-0-0-1": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 1, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7ce209c0-cf6e-4459-a01f-b5498a976bcf", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 243, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__001.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 35.5 }, "FrameKey-0-0-41": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 41, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f1774db8-84bf-4bfe-8335-4c2e03e62b61", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5011, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__041.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 41, "ZPositionUm": 55.5 }, "FrameKey-0-0-38": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 38, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0cbcb041-7145-4ea7-a3d4-0a5c048e987e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4649, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__038.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 38, "ZPositionUm": 54 }, "FrameKey-0-0-25": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 25, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28854e45-b823-4f63-aad2-8d5cfd214776", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3099, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__025.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 47.5 }, "FrameKey-0-0-48": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 48, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "350a98e1-02b1-494f-9f77-805551070435", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5841, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__048.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 48, "ZPositionUm": 59 }, "FrameKey-0-0-35": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:55 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 35, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "dbc2b357-63d8-4c16-b41b-6f0690f89ece", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4294, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__035.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 35, "ZPositionUm": 52.5 }, "FrameKey-0-0-46": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 46, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4b6e2f3b-1bc3-4a7c-bcce-a8137e1fd45e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5604, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__046.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 46, "ZPositionUm": 58 }, "FrameKey-0-0-23": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 23, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "44780ab1-9071-4e7a-9677-139fcc943bff", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2862, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__023.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 46.5 }, "FrameKey-0-0-6": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 6, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7b23941e-e3dc-46de-aead-30a2fab20d87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 840, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__006.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 38 }, "FrameKey-0-0-32": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 32, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "84353328-02ef-4454-ba6c-62e9c8285c1d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3935, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__032.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 32, "ZPositionUm": 51 }, "FrameKey-0-0-52": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 52, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db19be9f-1f07-472a-a7a9-d375f951ce6d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6317, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__052.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 52, "ZPositionUm": 61 }, "FrameKey-0-0-34": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 34, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "15d8c22d-87bc-485d-b48d-a576d69fb61f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4176, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__034.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 34, "ZPositionUm": 52 }, "FrameKey-0-0-27": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 27, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e74c60e5-df6e-4214-b22b-da8014d95269", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3342, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__027.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 48.5 }, "FrameKey-0-0-50": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 50, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f7fddf5b-7118-402e-91f3-f1c21bcfc8fc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6078, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__050.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 50, "ZPositionUm": 60 }, "FrameKey-0-0-5": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 5, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f02c7519-4bac-4790-889a-3d3415e8986f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 721, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__005.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 37.5 }, "FrameKey-0-0-59": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 59, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1caae8d0-7703-434a-a1e7-30dfdaf62709", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 7152, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__059.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 59, "ZPositionUm": 64.5 }, "FrameKey-0-0-19": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:53 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 19, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6c9d9d53-dc0b-4df7-a896-9943827d33c5", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2388, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__019.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 44.5 }, "FrameKey-0-0-28": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 28, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8907f3b6-6a4a-4b6c-929b-f003336b4582", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3460, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__028.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 49 }, "FrameKey-0-0-49": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 49, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8a2ede9c-d489-402a-af3c-bcf5d6bb9668", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5959, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__049.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 49, "ZPositionUm": 59.5 }, "FrameKey-0-0-47": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 47, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "37686406-436f-44e2-9d03-f7a48a0b3268", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5722, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__047.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 47, "ZPositionUm": 58.5 }, "FrameKey-0-0-51": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:56 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 51, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "ab34bdbe-48e3-40b8-a638-b8b026c15110", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6199, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__051.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 51, "ZPositionUm": 60.5 }, "FrameKey-0-0-33": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 33, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "429bfbe6-fa45-4cd3-a14f-5ed6086af6f2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4057, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__033.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 33, "ZPositionUm": 51.5 }, "FrameKey-0-0-29": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 29, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f5e9253d-65dd-44b0-9940-38e4fed0903d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3579, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__029.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 49.5 }, "FrameKey-0-0-54": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:57 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 54, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "98a77a0f-20d8-4879-a0ef-afa54a96c1df", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 6558, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__054.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 54, "ZPositionUm": 62 }, "FrameKey-0-0-17": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 17, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2aeda08b-ff80-4bb1-89c7-37428f411bdc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2146, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__017.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 43.5 }, "FrameKey-0-0-4": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:51 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 4, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "28f5901c-e549-4c91-ad01-44fc90ea4085", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 599, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__004.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 37 }, "FrameKey-0-0-10": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 10, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c42c0683-9dd9-4286-8702-913009cd5b58", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1317, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__010.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 40 }, "FrameKey-0-0-30": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:54 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 30, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a887c036-e502-4a00-b1ed-16b96e38132b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3698, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__030.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 50 }, "FrameKey-0-0-16": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 50, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "18", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-22 16:43:52 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 16, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5a76f56b-1abf-499d-9b62-b3337aaca5e4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2028, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f50c07f2-6d9c-4cb7-aeb3-3e4948efc549", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-22 16:43:51 -0400", "Date": "2014-07-22", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "EnderI5407_13DIV_ROI-01_brightfield", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "50.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__016.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 43 } }