culture: ender24728 age: 13 DIV temperature: 30C ROI: 01 white LED level: 20 blue LED level: -- exposure: 50ms x-position: .30953 y-position: 1.18033 z-position: 26.5 imaging notes: Ender24728_13DIV_ROI-02_no-drug_no-stim_3 --------------------- TIME: 10:30pm num_frames:2400 (target is 3min, 20s recording) length of image recording: 198.9658 s MCD file: ender24728_13DIV_no-drug_no-stim_0002.mcd imaging notes: Ender24728_13DIV_ROI-02_no-drug_no-stim_5 --------------------- TIME: 10:40pm num_frames: 4100 (target is 5min, 20s recording) length of image recording: 339.1508 MCD file: ender24728_13DIV_no-drug_no-stim_0007.mcd and ender24728_13DIV_no-drug_no-stim_0008.mcd (accidentally made these only 3min still) added vehicle at 10:45pm imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_1 --------------------- TIME: 10:55pm num_frames: 4100 (target is 5min, 20s recording) length of image recording: 339.2008 s MCD file: ender24728_13DIV_vehicle_no-stim_0002.mcd imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_2 --------------------- TIME: 11:05pm num_frames:2400 (target is 3min, 20s recording) length of image recording: 198.0074 s MCD file: ender24728_13DIV_vehicle_no-stim_0004.mcd imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_3 --------------------- num_frames: 4100 (target is 5min, 20s recording) length of image recording: 339.072 s MCD file: ender24728_13DIV_vehicle_no-stim_0008.mcd imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_4 --------------------- num_frames: 2400 (target is 3min, 20s recording) length of image recording: 199.187 s MCD file: ender24728_13DIV_vehicle_no-stim_0012.mcd imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_7 --------------------- num_frames: 4100 (target is 5min, 20s recording) length of image recording: 339.134 s MCD file: ender24728_13DIV_vehicle_no-stim_0023.mcd ender24728_13DIV_vehicle_no-stim_0024.mcd (accidentally made it 300s instead of 330) imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_8 --------------------- num_frames: 2400 (target is 3min, 20s recording) length of image recording: MCD file: imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_9 --------------------- num_frames: 2361 (failed early, was going to 4100) length of image recording: 195.7282 s MCD file: ender24728_13DIV_vehicle_no-stim_0030.mcd imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_10 --------------------- time: 1:30 AM num_frames: 3295 length of image recording: 272.9586 s MCD file: stopped recording MCRack to save space and limit errors imaging notes: Ender24728_13DIV_ROI-02_vehicle_no-stim_10 --------------------- time: 1:50 AM num_frames: 2400 length of image recording: 208.3231 s (lagging compared to usual!) So now we have recordings over the course of about ~4 hours to assess bleaching.