culture: ender24716
age: 13 DIV
temperature: 30C
ROI: 03

white LED level: 30
exposure: 50ms

x-position: 0.38610
y-position: 1.45814
z-position: 47.5

positioned over center electrodes (slight shifted more toward 44 and 45)


label is pretty faint.  it looks like we'll need to use LED=30 again.
i'll keep it down to 3-minute protocols to avoid too much bleaching by the end.

bursting is less frequent than other cultures (but that's good--more comparable to our other experiments)


imaging notes: Ender24716_ROI-03_no-drug_ChR2-stim_2
---------------------
num_frames: 2400
total time: 199.277 s
corresponding MCD file: Ender24716_ROI-03_no-drug_ChR2-stim_0002.mcd



imaging notes: Ender24716_ROI-03_no-drug_no-stim_2
---------------------
num_frames: 2400
total time: 199.174 s
corresponding MCD file: Ender24716_ROI-03_no-drug_no-stim_0002.mcd


ADDED CNQX at 12:00AM
recording for 10min on MEA
some spiking still, but not obvious bursts


imaging notes: Ender24716_ROI-03_CNQX_no-stim_2
---------------------
num_frames: 2400
total time:  198.9334s
corresponding MCD file: Ender24716_ROI-03_CNQX_no-stim_0002.mcd



imaging notes: Ender24716_ROI-03_CNQX_ChR2-stim_2
---------------------
num_frames: 2400
total time:  199.194 s
corresponding MCD file: Ender24716_ROI-03_CNQX_ChR2-stim_0002.mcd

stimulation kind of evoked a burst, but not super widespread
same deal with second stimulus--lots of spikes onf ch 45, then one or two on other channels, and not even all the bursters from before

i'll wait 5min then try another



imaging notes: Ender24716_ROI-03_CNQX_ChR2-stim_4
---------------------
time: 12:30am
num_frames: 2400
total time:  199.3504 s
corresponding MCD file: Ender24716_ROI-03_CNQX_ChR2-stim_0004.mcd


imaging notes: Ender24716_ROI-03_CNQX_ChR2-stim_6
---------------------
time: 12:45am
num_frames: 2400
total time:  199.283 s
corresponding MCD file: Ender24716_ROI-03_CNQX_ChR2-stim_0006.mcd