{ "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "FrameKey-0-0-3": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 3, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "9fa8966f-e930-4c80-b8ed-69cb0ba79112", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 366, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__003.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 33.5 }, "FrameKey-0-0-31": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 31, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "83f28df3-fdc5-450c-b640-f7a9c39905b7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2911, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__031.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 31, "ZPositionUm": 47.5 }, "FrameKey-0-0-43": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 43, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "fdc98a7b-131a-4750-b890-3b2354b0b8f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3997, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__043.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 43, "ZPositionUm": 53.5 }, "FrameKey-0-0-40": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 40, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "57dd251c-b2cd-4c38-84be-9f6afb0a8d28", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3725, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__040.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 40, "ZPositionUm": 52 }, "FrameKey-0-0-14": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 14, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "904f7f4a-4b34-4256-ac7f-61c0cba61ea1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1367, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__014.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 39 }, "FrameKey-0-0-55": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 55, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "433ea6f0-cc3c-44a9-ba86-950879a19005", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5091, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__055.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 55, "ZPositionUm": 59.5 }, "FrameKey-0-0-11": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 11, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "60bd2cca-1c05-447a-ae3a-d07aa079da34", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1095, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__011.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 37.5 }, "FrameKey-0-0-45": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 45, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d7b414ad-424d-429f-88ee-f4fb85ed9de0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4184, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__045.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 45, "ZPositionUm": 54.5 }, "FrameKey-0-0-37": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 37, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "18b4c07b-55f7-4dd8-a5ea-8e495623b692", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3453, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__037.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 37, "ZPositionUm": 50.5 }, "FrameKey-0-0-58": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 58, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a4992029-4ab7-4bd5-9731-d71dc6bdc939", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5363, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__058.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 58, "ZPositionUm": 61 }, "FrameKey-0-0-26": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 26, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "68d3c02d-071a-4ff7-aecf-d960a3dd0a30", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2458, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__026.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 45 }, "FrameKey-0-0-7": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 7, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b98528af-3b82-4ece-b35f-044cba4e86bd", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 728, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__007.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 35.5 }, "FrameKey-0-0-8": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 8, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "beea94d2-96e8-4490-93d5-d01da45cb6c7", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 819, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__008.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 36 }, "FrameKey-0-0-39": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 39, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3fcbab0e-5484-454a-ab9b-fec9708f37a4", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3634, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__039.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 39, "ZPositionUm": 51.5 }, "FrameKey-0-0-9": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 9, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "b1bfa9fd-6c95-4890-bfcc-c33cd44ac0d3", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 910, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__009.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 36.5 }, "FrameKey-0-0-60": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": null, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 60, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "79a1ed40-d877-420a-91a2-618dddde5e0b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5544, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__060.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 60, "ZPositionUm": 62 }, "FrameKey-0-0-13": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 13, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "e3eb8c96-0e3c-4b04-9d3b-e1903eee92c0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1276, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__013.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 38.5 }, "FrameKey-0-0-21": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 21, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "2d523e76-ce26-440c-b339-77353c3d8122", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2006, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__021.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 42.5 }, "FrameKey-0-0-53": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 53, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "db88f4f8-6681-4cf3-8fdb-faa2cbb79471", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4909, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__053.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 53, "ZPositionUm": 58.5 }, "FrameKey-0-0-2": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 2, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df104f9d-bab7-4962-a180-82b119b603db", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 275, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__002.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 33 }, "FrameKey-0-0-56": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 56, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "212174b3-3987-4fb0-8b82-04b873cad8e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5182, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__056.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 56, "ZPositionUm": 60 }, "FrameKey-0-0-0": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -15, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 0, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4ae17b29-9368-4f0b-9dd1-04808a8d9abc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 94, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": 0, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__000.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 32 }, "FrameKey-0-0-36": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 36, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "88b8aaa9-c723-4283-b8ee-6c9df9c54d74", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3362, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__036.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 36, "ZPositionUm": 50 }, "FrameKey-0-0-42": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 42, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "29e4b20c-707c-4dba-b225-654d6d223d6a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3906, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__042.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 42, "ZPositionUm": 53 }, "FrameKey-0-0-57": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 13.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 57, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "7863696d-2821-4a93-9be5-8313d9d5220e", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5272, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__057.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 57, "ZPositionUm": 60.5 }, "FrameKey-0-0-24": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 24, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "454a9a59-9659-4ef9-b035-29bb38537e8f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2276, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__024.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 44 }, "FrameKey-0-0-18": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 18, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d6acc697-a4d0-4413-9ca3-edbc3ce9216d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1731, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__018.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 41 }, "FrameKey-0-0-44": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 44, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6140307e-ce73-45e4-a3d7-75210bf8567d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4093, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__044.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 44, "ZPositionUm": 54 }, "FrameKey-0-0-20": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 20, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "01aab213-64c3-4126-a0ed-6d82ced5148f", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1913, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__020.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 42 }, "FrameKey-0-0-12": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 12, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "598a9184-cd1a-414d-a2b5-ca46e3ffe914", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1185, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__012.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 38 }, "FrameKey-0-0-15": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 15, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8466f55b-506a-4f11-b1f2-8f1b293871aa", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1458, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__015.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 39.5 }, "FrameKey-0-0-22": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 22, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "df4c540d-f243-4d78-a859-377361ea5a11", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2096, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__022.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 43 }, "FrameKey-0-0-1": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 1, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "607b06ca-c629-4c23-bd1e-cbd4d4b41558", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 184, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__001.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 32.5 }, "FrameKey-0-0-41": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 41, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "228ab410-76ef-4ebf-a6e6-bfb7d46dcde2", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3815, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__041.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 41, "ZPositionUm": 52.5 }, "FrameKey-0-0-38": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 4, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 38, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "d1ec04b4-371d-466d-a159-4813aa73dcd6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3544, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__038.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 38, "ZPositionUm": 51 }, "FrameKey-0-0-25": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 25, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c89131ba-bd56-49cd-8ca6-bcb48060076b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2367, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__025.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 44.5 }, "FrameKey-0-0-48": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 48, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "be1dfaf1-d73c-4878-b177-7820528ba6a1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4456, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__048.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 48, "ZPositionUm": 56 }, "FrameKey-0-0-35": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 35, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "10cfc40e-1078-4c66-8915-0affd7165e25", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3272, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__035.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 35, "ZPositionUm": 49.5 }, "FrameKey-0-0-46": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 46, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "4a1562dc-807c-44c7-ae7f-c9997838de8a", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4275, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__046.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 46, "ZPositionUm": 55 }, "FrameKey-0-0-23": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -3.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 23, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8355af99-2ca5-4f0a-b412-13139e9816a6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2186, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__023.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 43.5 }, "FrameKey-0-0-6": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 6, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "34b6bae8-5197-4f5d-8b10-c530b2b6d6d1", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 638, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__006.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 35 }, "FrameKey-0-0-32": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 32, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c3e02c94-c6f6-4cad-8e7f-fe514832b15b", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3001, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__032.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 32, "ZPositionUm": 48 }, "FrameKey-0-0-52": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 11, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 52, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "f6f815ff-8bb1-49aa-87b6-bba387722128", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4819, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__052.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 52, "ZPositionUm": 58 }, "FrameKey-0-0-34": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 2, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 34, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "c1edb94b-8ae7-4877-a753-11c469d719e0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3182, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__034.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 34, "ZPositionUm": 49 }, "FrameKey-0-0-27": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 27, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6a13c880-fc26-4b8e-98de-52a9f81c89fe", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2549, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__027.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 45.5 }, "FrameKey-0-0-50": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 50, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a75f26b5-0dd6-4d3e-932b-94ff9fdefdfc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4638, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__050.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 50, "ZPositionUm": 57 }, "FrameKey-0-0-5": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -12.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 5, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "6ab666e0-6546-44fe-b4ab-d4bc7e812468", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 548, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__005.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 34.5 }, "FrameKey-0-0-59": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 14.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 59, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "8d33ff21-a100-463c-920d-9673b382eb00", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 5453, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__059.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 59, "ZPositionUm": 61.5 }, "FrameKey-0-0-19": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -5.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 19, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5c92f3d6-780a-41bb-8820-63acc9eb491c", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1822, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__019.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 41.5 }, "FrameKey-0-0-28": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -1, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:00 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 28, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "740f4db0-e48f-4b85-9211-a364c6227713", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2639, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__028.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 46 }, "FrameKey-0-0-49": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 9.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 49, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "1906aa58-41dd-4d0b-bf9f-47c18dc67fd8", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4547, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__049.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 49, "ZPositionUm": 56.5 }, "FrameKey-0-0-47": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 8.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:02 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 47, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "eb20695e-65af-4dff-a0ce-c68c83aa21f0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4365, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__047.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 47, "ZPositionUm": 55.5 }, "FrameKey-0-0-51": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 10.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 51, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "0623c902-64fd-485b-b45c-72f36b531ef6", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4729, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__051.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 51, "ZPositionUm": 57.5 }, "FrameKey-0-0-33": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 1.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 33, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "17ed29e7-da1c-4ee1-b5eb-7a97f7e52611", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 3091, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__033.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 33, "ZPositionUm": 48.5 }, "FrameKey-0-0-29": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -0.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 29, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "106acb59-c1b4-44a7-b4c7-4c6d3b907ccc", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2730, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__029.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 46.5 }, "FrameKey-0-0-54": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 12, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:03 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 54, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "aeab071e-e0a6-4a49-a6c0-8e243dea8d87", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 4999, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__054.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 54, "ZPositionUm": 59 }, "FrameKey-0-0-17": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -6.5, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 17, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "5dd6407b-0328-4a48-99d2-9cdae244fbe0", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1640, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__017.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 40.5 }, "FrameKey-0-0-4": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -13, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:58 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 4, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "bb10feeb-699f-40bd-9a26-f27ff4b2130d", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 457, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__004.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 34 }, "FrameKey-0-0-10": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -10, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 10, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "a699a212-3646-4a86-86e2-d8cd5e2bb445", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1005, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__010.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 37 }, "FrameKey-0-0-30": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": 0, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:19:01 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 30, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "244ea7d4-703a-424c-8ff2-0140cf560970", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 2821, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__030.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 47 }, "FrameKey-0-0-16": { "Core-Focus": "PIZStage", "Channel": null, "Core-Initialize": "1", "COM7-AnswerTimeout": "500.0000", "COM7-Handshaking": "Off", "FrameIndex": 0, "quantEM-ChipName": "QuantEM:512SC", "quantEM-Port": "EM", "PIZStage-StepSizeUm": "0.0100", "quantEM-OutputTriggerFirstMissing": "0", "Camera": "", "quantEM-ShutterOpenDelay": "0", "quantEM-Firmware Version": "2052", "quantEM-Offset": "0", "Core-ImageProcessor": "", "SlicePosition": -7, "quantEM-PreampOffLimit": "10000", "Exposure-ms": 20, "quantEM-MultiplierGain": "1", "quantEM-ReadoutRate": "10MHz 16bit", "COM7-Description": "Serial port driver (boost:asio)", "quantEM-ActualInterval-ms": "0.0000", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "PIZStage-MaxZ_um": "100.0000", "quantEM-Binning": "1", "quantEM-CCDTemperature": "26", "COM7-Parity": "None", "quantEM-Name": "PM1394Cam00", "quantEM-PrescanPixels": "28", "quantEM-PreampDelay": "1", "NextFrame": 0, "Core-XYStage": "", "AxisPositions": null, "quantEM-PostscanPixels": "12", "quantEM-ShutterCloseDelay": "0", "PIZStage-Interface": "Remote: Interface commands mode", "Time": "2014-07-19 23:18:59 -0400", "quantEM-ShutterMode": "Open Shutter Pre-Exposure", "quantEM-PVCAM Version": "2.7.7", "COM7-Verbose": "1", "quantEM-Y-dimension": "512", "Core-AutoFocus": "", "COM7-StopBits": "1", "quantEM-Trigger Timeout (secs)": "2", "quantEM-ClearCycles": "2", "quantEM-ClearMode": "Clear Pre-Exposure", "quantEM-X-dimension": "512", "BitDepth": 16, "Slice": 16, "quantEM-Description": "PVCAM API device adapter", "ChannelIndex": 0, "PIZStage-WaitForResponse": "No", "quantEM-PMode": "Frame Transfer Parallel Clocking", "Core-Shutter": "", "COM7-BaudRate": "9600", "quantEM-MaskLines": "537", "UUID": "3d0e729b-9445-4c62-9575-c1904ad81e91", "PIZStage-Name": "PIZStage", "Core-Camera": "quantEM", "PIZStage-Description": "Physik Instrumente (PI) E-662 Adapter", "Core-TimeoutMs": "5000", "Core-SLM": "", "quantEM-Gain": "3", "ElapsedTime-ms": 1549, "quantEM-CCDTemperatureSetPoint": "-30", "Frame": 0, "quantEM-FullWellCapacity": "240000", "Core-ChannelGroup": "", "PositionIndex": 0, "Width": 512, "WaitInterval": null, "PositionName": null, "PixelSizeUm": 0, "Height": 512, "Summary": { "Slices": 61, "Interval_ms": 0, "UUID": "f292cef1-78dd-49d0-9a8e-7d12aa34143a", "UserName": "mkuykendal", "Depth": 2, "PixelType": "GRAY16", "Time": "2014-07-19 23:18:58 -0400", "Date": "2014-07-19", "z-step_um": 0.5, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [2147483647], "Width": 512, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20120930", "ROI": [ 0, 0, 512, 512 ], "ChNames": ["Default"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 512, "GridColumn": 0, "Prefix": "Ender24716_ROI-02_brightfield_zstack", "PixelSize_um": 0, "Frames": 1, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "falcon-cal-imag", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [-1], "TimeFirst": false, "ChContrastMax": [-2147483648], "Positions": 1, "Directory": "" }, "Source": "quantEM", "quantEM-TransposeXY": "0", "Binning": "1", "quantEM-TransposeMirrorY": "0", "PixelType": "GRAY16", "quantEM-TransposeMirrorX": "0", "quantEM-Exposure": "20.00", "quantEM-PixelType": "16bit", "PIZStage-Port": "COM7", "COM7-Name": "COM7", "COM7-DataBits": "8", "YPositionUm": null, "quantEM-TransposeCorrection": "0", "XPositionUm": null, "quantEM-TriggerMode": "Timed", "FileName": "img_000000000__016.tif", "PIZStage-Position": "0.0000", "Core-Galvo": "Undefined", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 40 } }