culture: dingo25307
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 20
blue LED level: 75
exposure: 50ms

x-position: -0.52769
y-position: 1.45814
z-position: 46um


channel 78 is bad.  exclude from analysis.


imaging notes: coolio25307 quicktest led 15
---------------------
num_frames: 441
total time: 38.8912 s
corresponding MCD file: 

imaging notes: coolio25307 quicktest led 25
---------------------
num_frames: 350
total time: 30.45 s
corresponding MCD file: 


I think we're going to need 30 on this on.  Will shorten protocols to 3 minutes (with 10s before and after), where blue light goes on at 30s, then 2min pass, then blue light goes on again (so a total of 3min, 20s)



imaging notes: Dingo25307_ROI-01_Rhod-3_no-drug_ChR2-stim_1
---------------------
num_frames: 
total time: 
corresponding MCD file: 
i forgot to increase time to 3 minutes on imaging recording (that sucks because it means i was bleaching for 2.5min when i didn't have to be).  we'll try again here...



imaging notes: Dingo25307_ROI-01_Rhod-3_no-drug_ChR2-stim_2
---------------------
num_frames: 2400
total time: 198.64 s
corresponding MCD file: dingo25307_ROI-01_no-drug_ChR2-stim_0003.mcd



imaging notes: Dingo25307_ROI-01_Rhod-3_no-drug_no-stim_1
---------------------
num_frames: 2400
total time: 198.8414 s
corresponding MCD file: dingo25307_ROI-01_no-drug_no-stim_0002.mcd



ADDED CNQX AT 9:15PM
looks like there was 1 or 2 bursts during 10min after CNQX was added, 


imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_no-stim_1
---------------------
num_frames: 2400
total time: 199.497 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0003.mcd



imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_ChR2-stim_1
---------------------
num_frames: 2400
total time: 199.497 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0003.mcd

first stim evoked some spikes, but second stim evoked a real looking burst



imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_ChR2-stim_2
---------------------
num_frames: 2400
total time: 199.172 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0006.mcd

first burst was good!
there was a burst just a little before second stim, so not sure if that did much



imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_ChR2-stim_3
---------------------
num_frames: 2400
total time: 199.301 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0010.mcd

natural bursts happened within 20sec before both stimuli.... those stim-evoked burst prob shouldn't be counted anyway since (if we were doing this the right way) we wouldn't even be delivering a stimulus then (since the controller would be satisfied and not stimulate)



imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_ChR2-stim_4
---------------------
num_frames: 2400
total time: 199.092 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0013.mcd

first burst was good.
second burst was good.


imaging notes: Dingo25307_ROI-01_Rhod-3_CNQX_ChR2-stim_5
---------------------
num_frames: 2400
total time: 198.678 s
corresponding MCD file: dingo25307_ROI-01_CNQX_ChR2-stim_0016.mcd

first burst was great!  no bursts for entire recording before that.
left before second, so not sure.


sample looks pretty bleached.  i'm gonna stop till the next one and transfer some files.