culture: coolio25020 age: 13 DIV temperature: 30C ROI: 01 white LED level: 20 (unless otherwise specified in filename) blue LED level: 75 exposure: 50ms x-position: 0.89837 y-position: 0.34989 z-position: 38um positioned over 67, 77, 68, and 78 (all fairly active before experiment, and 67 responsive to light) recording for 5min, with 10s before and after note: in previous recordings, i noticed there there's something wrong with channel 37 and noise. this recording had really bad noise on all channels to start, and i spent ~20min fiddling with it. imaging notes: coolio25020_ROI-01_quick-test_exp-50ms_LED-20 --------------------- num_frames: 350 total time: 30.4722 s corresponding MCD file: none imaging notes: coolio25020_ROI-01_no-drug_ChR2-stim_2 --------------------- num_frames: 4100 total time: ?? s corresponding MCD file: coolio25024_ROI-01_no-drug_ChR2-stim_0004.mcd error 2min into recording (in MC-Rack) redo... imaging notes: coolio25020_ROI-01_no-drug_ChR2-stim_3 --------------------- num_frames: 4100 total time: 339.6064 s corresponding MCD file: coolio25024_ROI-01_no-drug_ChR2-stim_0006.mcd imaging notes: coolio25020_ROI-01_no-drug_no-stim_1 --------------------- num_frames: 4100 total time: 339.5634 s corresponding MCD file: coolio25024_ROI-01_no-drug_no-stim_0002.mcd same stimulus file except that I've turn the light off (but digital pulses will still be there) ****** ADDED 40uM CNQX AT 9:42PM ****** recording for 10min 30 sec afterwards clearly still synchronous activity imaging notes: coolio25020_ROI-01_CNQX_no-stim_1 --------------------- num_frames: 4100 total time: 338.55s corresponding MCD file: coolio25024_ROI-01_CNQX_no-stim_0002.mcd again, same stimulus file except that I've turn the light off (but digital still there)pulses some bursting... maybe less than before though? imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_2 --------------------- num_frames: 4100 total time: 338.846 s corresponding MCD file: coolio25024_ROI-01_CNQX_ChR2-stim_0002.mcd now I've turned blue light back on (ready to be triggered) we have extra time, so let's do another CNQX and another CNQX+stim recording... imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_2 --------------------- num_frames: 4100 total time: 339.5274s corresponding MCD file: coolio25024_ROI-01_CNQX_no-stim_0005.mcd I've turn the light off (but digital pulses are still there) imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_5 --------------------- blue light back on (ready to be triggered) num_frames: 4100 (aborted at 1167) total time: aborted at 97.7384 corresponding MCD file: coolio25024_ROI-01_CNQX_ChR2-stim_0005.mcd the light stimulation doesn't seem to be evoking much of anything... at least at 60s. i aborted this early. manually flipping switch on seems to do the trick. however, i'm only flashing for 10ms (to be consistent with NR recordings). let me try turning up light power... imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_13 --------------------- blue light back on (ready to be triggered), and power turned up to max (99) num_frames: 4100 total time: 339.831 corresponding MCD file: coolio25024_ROI-01_CNQX_ChR2-stim_0013.mcd the light stimulation doesn't seem to be evoking much of anything... at least at 60s. i aborted this early. trying one more thing with pulse width at 50ms imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_16 --------------------- blue light back on (ready to be triggered), and power turned up to max (99), and pulse width extended to 50ms num_frames: 4100- aborted at 977 total time: 81.9641 s corresponding MCD file: coolio25020_ROI-01_CNQX_ChR2-stim_0016.mcd still not doing anything... imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_18 --------------------- blue light back on (ready to be triggered), and power turned up to max (99), and pulse width extended to 200ms num_frames: 4100- aborted at 1022 total time: 86.3032 s corresponding MCD file: coolio25020_ROI-01_CNQX_ChR2-stim_0018.mcd there was a burst, but i think it might have been just right before light pulse. let's try again... imaging notes: coolio25020_ROI-01_CNQX_ChR2-stim_21 --------------------- blue light back on (ready to be triggered), and power turned up to max (99), and pulse width extended to 200ms num_frames: 4100- aborted at 1022 total time: 86.3032 s corresponding MCD file: coolio25020_ROI-01_CNQX_ChR2-stim_0021.mcd okay blue light definitely did nothing. on this last one. i'm depressed. well, let's try turning on the camera light just a few seconds before blue light stim 10ms didn't work 200ms did work. #28: 39.8754s, 468 frames