culture: Bacon24729
age: 14 DIV
temperature: RT
ROI: 02

encompasses center electrodes
(basically same as ROI-01 except now I'm doing long recordings and I've made me z close to cell layer)

LED level: 25 (white, through Cy3 filter)
exposure: 50ms

x-position: 0.38733
y-position: 1.00710
z-position: 75um


imaging notes: 1
---------------------
exposure: 50 ms
num_frames: 8000 (note: LED will be on for exactly 5min, so even if this is too many frames, we'll know when 10min ends; also light-off time can give us a sense of camera black level)
total time: 661.3498 s
corresponding MCD file: Bacon24729_ROI-02_0002.mcd

fluorescence went down at 601.9904s, frame 7280
so let's do 7500 frames in subsequent 10min recordings






w/ TTX

tried to go back to same position, this is how close i got...
y-position: 1.00710
z-position: 75um

imaging notes: 2
---------------------
exposure: 50 ms
num_frames: 7500
total time: 653.4714 s
corresponding MCD file: Bacon24729_ROI-02_0004.mcd

fluorescence went down at 595.8776s, frame 6836

NOTE: accidentally did this as timed recording instead of triggered. can still get info from when light turns on though (this goes for ROI-4 in ttx also)








got to the same as starting position:
x-position: 0.38733
y-position: 1.00710
z-position: 75um

imaging notes: 3
---------------------
ERROR PARTWAY THROUGH!



imaging notes: 4
---------------------
exposure: 50 ms
num_frames: 7500
total time: 650.7525 s
corresponding MCD file: Bacon24729_ROI-02_0008.mcd

fluorescence went down at 601.2369s, frame 6929

accidentally did a timed recording again.  doh!