culture: Bacon24729
age: 14 DIV
temperature: RT
ROI: 01

encompasses center electrodes


imaging notes: 1
---------------------
exposure: 100 ms
num_frames: 450
total time: 60.9571 s
light: white LED @ 30
z-location: 11 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0004.mcd

obvious burst in middle


imaging notes: 2
---------------------
exposure: 100 ms
num_frames: 450
total time: 60.8781 s
light: white LED @ 30
z-location: 16 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0006.mcd

a few bursts


imaging notes: 3
---------------------
exposure: 100 ms
num_frames: 450
total time: 61.5331 s
light: white LED @ 30
z-location: 23 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0008.mcd

a few obvious bursts

(#4 aborted--wrong light)


imaging notes: 5
---------------------
exposure: 50 ms
num_frames: 450
total time: 38.3236 s
light: white LED @ 30
z-location: 11 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0012.mcd

burst around 20s


imaging notes: 6
---------------------
exposure: 50 ms
num_frames: 450
total time: 41.0186 s
light: white LED @ 30
z-location: 11 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0014.mcd


imaging notes: 7
---------------------
exposure: 50 ms
num_frames: 450
total time: 38.4256 s
light: white LED @ 30
z-location: 11 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0016.mcd


TURNED LED DOWN TO 25

imaging notes: 7
---------------------
exposure: 50 ms
num_frames: 450
total time: 41.2086 s
light: white LED @ 25
z-location: 11 um
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon24729_ROI-01_0018.mcd

While some cells are still saturating during burst, this is better and i'll leave it at this for remainder of experiment