culture: Bacon22564
age: 13 DIV
temperature: 35C
ROI: 6

encompasses electrodes 56, 66, 57, 67


imaging notes: 1
---------------------
exposure: 50 ms
num_frames: 710
total time: 61.702 s
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0002.mcd

only one spk the whole time


imaging notes: 2
---------------------
exposure: 50 ms
num_frames: 710
total time: 61.695 s
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0004.mcd

only three spk the whole time


imaging notes: 3
---------------------
exposure: 100 ms
num_frames: 450
total time: 100+ seconds...
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0009.mcd

small burst (only 3 channel participating though)

but only 20 frames had finished by 1min... going to restart the camera...



imaging notes: 5
---------------------
exposure: 100 ms
num_frames: 450
total time: 61.0411 s
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0014.mcd

small burst (5 channels) around 20s



imaging notes: 6 - attempting 5min rec
---------------------
exposure: 100 ms
num_frames: 2250
total time:  299.8911 s
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0016.mcd

1 spk, but i forgot to extend the mc-stim light/recording time to 5 min (instead of 1 min)


imaging notes: 7 - 5min rec
---------------------
exposure: 100 ms
num_frames: 2250
total time:  299.6641 s
light: white LED @ 30
filter: Cy3 (Rhod-3)
corresponding MCD file: Bacon22564_ROI-06_37C_0018.mcd

not much spiking.  maybe raising temp isn't good?


okay, i'm going to test blue light stimulation now

i set blue LED to 70 for this test
10ms pulse of light at 1min
10ms pulse of light at 4min
MEA recording lasted 5min