O Statistics;Gr_Se_Sw of Sw (Group_Series_Sweep):;Currently active group, series and sweep number within the data tree. The total number of sweeps per series is given after of.
O Measure;Measure:;Allows to measure amplitudes and durations. With this option the user can measure the displayed data by pointing and dragging the mouse
O Scan;Scan:;Allows to measure signal amplitudes by scanning the individual data points. When selected, a marker is displayed on the trace. This marker can be moved forward and backward by dragging with the mouse
O OsciWindow;Zooming - Lassoing;It is possible to set the display scaling in the Oscilloscope window  by "lasso-ing" a screen region while pressing the left mouse button. When you release the mouse button, the marked area will be set to fill the oscilloscope screen. The scaling has to be done for each trace separately.
O Freeze;Freeze:;Freezes the present content of the oscilloscope. Any change in display parameters, such as scaling etc. will unfreeze the display again, as does a "Wipe" or switching to the amplifier window.
O Wipe;Wipe:;Clears the Oscilloscope completely.
O Repaint;Repaint:;Clears the Oscilloscope first, then redraws the last displayed trace. This is useful e.g., if you had the Overlay Sweep option activated, but then decided that you only want to see the last sweep of the series.
O OverlSwp;Overl. Swp.:;Displays all sweeps of a series without erasing the screen in between sweeps. The next series, however, will erase the screen.
O OverlSer;Overl. Ser.:;Displays all incoming sweeps without erasing the screen. This allows sweeps of different series to be computed. The screen can be wiped by pressing Backspace.
O DispTraceCol;Trace Color:;Displays the color of the selected trace
O DispTrace;Trace 1..16:;Selects the trace to which the scaling setting applies. The color of the selected trace is displayed above the Trace control.
O DFilter;Dig. Filter:;Shows the currently selected bandwidth of a digital non-lagging Gaussian low-pass filter (i.e. software filter). The -3dB cutoff frequency is specified in Hertz. It must be larger than 0.01 times the sampling rate. The filter is used for display purposes only; no changes to the data are performed.
O YScale;Y-scale:;For each trace you can control the display scaling. The value of 1 corresponds to full scale of +/-10.24 V. This scaling does not affect the display of test pulses.
O YOffset;Y-offs.:;For each trace you can control the offset of the zero line. Offsets of traces may be between -1 and 1 relative to full scale of display (default = 0).
O YCenter;(><):;Clicking on the (><) button (or . on the numeric keypad) automatically centers the respective trace on the screen.
O Xmin;Start Time / End :;Section of the sweep to be shown on the screen in % (Start - End). The reset button (R) sets the full sweep length (0 to 100 %).
O Xmax;Start Time / End :;Section of the sweep to be shown on the screen in % (Start - End). The reset button (R) sets the full sweep length (0 to 100 %).
O Page;Page:;Page of display during replay of continuous data sweeps or when time axis is chosen to be less than 100%. Clicking on the right/left arrow control will display the next/previous page of the current sweep. Dragging the page number scrolls the data forward or backward; entering a page number will display that particular page. Page is highlighted whenever there is more than one page available.
E Monitor;Monitor:;Switch to monitoring mode. Only essential controls are displayed in this mode
E Tune;Tune:;Switch to tuning mode.
E ShowAll;ShowAll:;All available amplifier controls are displayed in this mode
E Macro1;SET-UP:;Resets all parameters (with the exception of LJ and Vo), and defines the parameters of the test pulse. Should be set after the pipette has entered the bath.
E Macro2;ON-CELL:;Switches the gain range to a typical setting for a cell-attached patch recording, sets initial C-fast estimates, and invokes an Auto C-fast compensation.
E Macro3;WHOLE-CELL:;Switches the gain range to a typical setting for a whole-cell recording, sets initial C-slow estimates, and invokes an Auto C-slow compensation.
E Gain;Gain:;Sets the scaling of the current monitor output.
E VHold;V-membrane / I-membrane :;Sets the desired holding voltage in voltage clamp mode and the holding current in current clamp mode.
E IHold;V-membrane / I-membrane :;Sets the desired holding voltage in voltage clamp mode and the holding current in current clamp mode.
E TstDacToStim1;DA-0 to Stim-1: ON;In case multiple amplifiers should be stimulated simultaneously, then the following has to be done: First use the DA-0 channel as DA-channel in the stimulation template of the pulse generator sequence. Second, select this option in the Amplifier window, to apply the DA-0 output to the Stim channel of the amplifier. This procedure allows simultaneous stimulation of multiple amplifiers on basis of a single sample of the digitizer clock. This is very important e.g. for studying gap junctions.
E Ipip1;I-mon:;Displays the DC current monitor signal.
E Vmon1;V-mon:;Displays the actually measured pipette voltage after correcting for liquid-junction potentials and offsets (provided the zero-current potential has been set correctly). This may differ (temporarily) from the holding voltage (e.g., during long stimulation pulses) as it indicates the average sum of V-membrane and the scaled stimulus voltage.
E Rmemb1;R-memb:;The seal resistance R-memb is determined from the current sampled during the baseline and the second half of the test pulse. R-memb can be encoded into a tone using the Sound feature.
E TestAdc;;The Input ADC selection determines which AD-channel is displayed in the oscilloscope.
E Mode;Mode:;Patchmaster tries to use the physiological convention whenever stimulating or acquiring data: therefore, a downward current always reflects a cationic influx into the cell. However, for this to work properly you have to always instruct the program about the actual recording configuration.
E PulseOff;Off:;Turns the test pulse off.
E PulseMode;double / single / TestSeries / Enter Name:;The two built-in test pulses can be selected from the test pulse pop-up menu: single or double. In this case mono- or bipolar pulse patterns are generated, output, and the current signal is sampled and displayed.
E NoiseOn;Noise:;If selected, the rms noise is continuously measured and updated in the field usually labeled R-memb. For the determination of the noise level, no pulses are output and current is sampled via the active AD-channel using the current filter settings (usually the Current Monitor 2 output, so that the bandwidth is determined by Filter 2). It is sampled in subsections of 10 times 256 points with a sample interval of 100 µs, i.e., a total length of 256 ms.
E PulseTrace;both/current/voltage:;Displays the chosen signal traces.
E PulseAmp;Amplitude/Length:;Amplitude and duration of built-in test pulses (single and double) can be specified in the dialog. The minimum pulse duration is 0.1 ms. No analysis of these pulses is provided, which makes the repetition interval quite short.
E PulseDur;Amplitude/Length:;Amplitude and duration of built-in test pulses (single and double) can be specified in the dialog. The minimum pulse duration is 0.1 ms. No analysis of these pulses is provided, which makes the repetition interval quite short.
E IScale;I-scale, V-scale:;Here you can change the display scaling of current and voltage for the Test Pulse.
E VScale;I-scale, V-scale:;Here you can change the display scaling of current and voltage for the Test Pulse
E LJunc;LJ:;Liquid Junction is a variable, to be set by the user, which allows to correct for liquid junction potentials and other offsets. It works in conjunction with the Vo operation. An online correction requires an Auto-Vo operation to be performed before seal formation and LJ to be set to an appropriate value. No correction is performed if LJ = 0.
E Vzero;Vo:;Displays the offset voltage (a voltage that is added to V-membrane to obtain the pipette command voltage). It can be set either by the Auto-Vo operation or by manually dragging the mouse after clicking into the item.
E AutoZero;Auto-Vo:;The Auto-Vo button calls a procedure for automatic zeroing of the pipette current. Thereby, an offset voltage (Vo) to the pipette potential is systematically varied until pipette current is zero. Range of Vo is +/- 200 mV. Auto-Vo is typically performed before seal formation. It only works when a pipette is inserted into the bath.
E SearchMode;Track:;This option implements a Search Mode, which is essentially a Auto-Vo procedure at a holding potential of 0 mV. The rate at which Auto-Vo is performed is determined by the entry Search Mode Delay in the EPC 10 menu.
E CFastTot;C-fast:;This is the total C-fast value.
E CFastTau;tau-fast:;This determines the time constant of C-fast (up to 8 µs). The value of tau-fast may be adjusted by dragging the mouse, or typing, or automatically by selecting the Auto function.
E CFast2;<>:;This will select both, amplitude (vertical) and time constant (horizontal) of C-fast. Values for each may be changed manually depending on whether the mouse is moved horizontally or vertically.
E AutoCFast;Auto C-fast:;Selection of this button performs an automatic compensation of C-fast and tau-fast. The procedure uses a routine that applies a number of small pulses (5 mV), averages the resulting currents and fits an exponential to deduce the capacitance compensation values required to cancel the current.
E CSlowRange;Range:;Selects the range for slow capacitance compensation
E CSlow;C-slow:;This is used to cancel slow capacitive currents that charge the cell membrane in the whole-cell configuration. The 30, 100 and 1000 pF ranges actually allow capacitance values to be compensated in the ranges of 0.12-30 pF, 0.4-100 pF and 4-1000 pF, respectively.
E RSeries;R-series:;Adjusts the resistance in series with the slow capacitance (range: 0.1 MOhm - 10 GOhm) to determine the time constant of the C-slow transient and for Rs-compensation. Adjustment is limited by the capacitance values and the range as described above. The value can be changed manually by dragging the mouse, or typing, or automatically by clicking on Auto.
E CSlow2;<>:;This will select both C-slow and R-series. Values for each are changed depending on whether the mouse is moved horizontally or vertically.
E AutoCSlow;Auto C-slow:;Selecting this function performs an automatic compensation of C-slow and R-series. The procedure uses a routine that applies short trains of square-wave pulses (number and amplitude of these pulses are specified in the EPC 10 menu by C-slow Num. Cycles and C-slow Peak Amplitude), averages the resulting currents, and fits an exponential to deduce the compensation values required to cancel the current.
E CapTrack;Cap. Track:;Selecting this button will perform repetitive Auto C-slow compensation. Clicking on Cap.Track again will turn it off.
E Delay;Delay:;This determines how many seconds to wait before the next auto-compensation is started. With short delays (e.g., 1 ms), membrane capacitance will be tracked at rates of >10 Hz (depending on the computer hardware).
E RsMode;;The series resistance compensation corrects for membrane voltage errors under conditions of high access resistance between pipette and cell interior. The amount of compensation can be changed by dragging the mouse or typing (range 0-95% ). The compensation is based on the value of R-series and will be effective only when Rs-comp is not Off, i.e., set to a speed value.
E RsComp;;The series resistance compensation corrects for membrane voltage errors under conditions of high access resistance between pipette and cell interior. The amount of compensation can be changed by dragging the mouse or typing (range 0-95% ). The compensation is based on the value of R-series and will be effective only when Rs-comp is not Off, i.e., set to a speed value.
E AutoRsComp;;A click on the Auto button automatically adjust the Rs-compensation setting to optimize the speed of a voltage jump at the cell membrane. The procedure tries to keep overshooting response to a minimum, since such overshoot leads to increased noise and introduces higher frequency components into the current signal.
E GLeak;;This controls a hardware leak compensation which uses the inverted and scaled stimulus signal and adds it to the current monitor outputs. Leak can be adjusted manually by dragging the mouse, or typing, or automatically by clicking on Auto. The maximal values attainable depend on the Gain Range. They are 2 nS, 200 nS and 20 µS for high, medium, and low ranges, respectively.
E AutoGLeak;Auto:;Automatically determines and corrects the leak conductance.
E TrackGLeak;Track:;Continuously updates the leak compensation.
E Filter1;Filter 1:;Controls an analog 3-pole filter in the current monitor pathway.
E Filter2;Filter 2:;Controls an analog 4-pole filter for current monitor 2 (Imon-2). Filter 2 is in series with Filter 1. Dragging the mouse or typing allows fine adjustment from 0.1-16 kHz in 0.1 kHz steps (guaranteed accuracy 0.5-15 kHz).
E F2Response;Filter 2:;Controls an analog 4-pole filter for current monitor 2 (Imon-2). Filter 2 is in series with Filter 1. Dragging the mouse or typing allows fine adjustment from 0.1-16 kHz in 0.1 kHz steps (guaranteed accuracy 0.5-15 kHz). Two filter characteristics can be selected from the pop-up menu
E StimFilter;Stim:;The stimulus can be filtered (2-pole Bessel) to reduce the amplitude of fast capacitance transients when the speed of potential changes is not critical.
E ExtScale;External:;The external Stim.Input is scaled by an editable factor (range: +/-0-10x), to allow for different external stimulators. It is strongly recommended to set External to Off (i.e., equal to zero) if no external stimulator is connected to the Stim.Input. This will prevent pick-up of external noise.
E Macro4;Macro button:;user definable macro button.
E Macro5;Macro button:;user definable macro button.
E Macro6;Macro button:;user definable macro button.
E Macro7;Macro button:;user definable macro button.
E Zap;Zap:;A high-voltage pulse is applied to the pipette in order to rupture the patch membrane. The parameters of the Zap pulse (duration and amplitude) can be specified in the lower part of the Amplifier window.
E SoundOn;Sound:;If this control is On, a sound is played proportional to R-membrane.
E Reset;Reset:;Selecting this button will reset the EPC 10 to its initial default configuration. It will reset the DA-channels to zero, which is useful e.g. to cancel the output of C-slow and R-series set by Cap.Track. In addition, Reset is very useful to define the initial state of the EPC 10 when recording a macro.
E MacroRecord;Record:;% Record %corresponds to Start Recording of the Macro %menu. To start macro recording, click on the Record %button. Then, perform all desired actions (the Notebook %window will print a protocol of the macro actions.) You may record %up to 50 actions. To specify a parameter value, enter it as usual %by dragging or keyboard entry.
E MacroLoad;Load:;% Loads the macro file whose name you can enter in the blue field. %
E ZapAmpl;Zap:;Sets the parameters for the Zap pulse, namely Amplitude and Duration. A ZAP pulse is a simple, square voltage pulse of a given amplitude and duration at the Stim-DA of the active amplifier. These 2 values are stored in the Configuration file. The ZAP amplitude can also be a negative value.
E ZapDurItem;Zap:;Sets the parameters for the Zap pulse, namely Amplitude and Duration. A ZAP pulse is a simple, square voltage pulse of a given amplitude and duration at the Stim-DA of the active amplifier. These 2 values are stored in the Configuration file. The ZAP amplitude can also be a negative value.
E ListState;List State:;Sends the actual amplifier state information to the Notebook.
E OnePulse;One Pulse:;As the name suggests, this button executes one test pulse. This is useful when test pulses are off and simply one test pulse is to be outputted.
E SaveRpip;R-memb -> R-pip:;Copies the value of R-memb into R-pip. This is used to store the pipette resistance in the data file before forming a seal.
E LFVCVmemb;LFVC V-memb:;Sets the membrane potential used for low-frequency voltage clamp.
E VmembtoLFVCVmemb;V-memb / V-mon:;Copies the values of V-memb respectively V-mon into LFVC V-memb.
E VmontoLFVCVmemb;V-memb / V-mon:;Copies the values of V-memb respectively V-mon into LFVC V-memb.
E LFVCVmembTau;Low Freq. VC:;Here you can select the speed of the feedback for Low-Frequency Voltage Clamp.  The low frequency voltage clamp mode is a modified current clamp mode that allows for the measurement of potential deflections, such as action potentials or synaptic potentials, while the average potential is kept constant at a value chosen by the user (LFVC V-memb). The circuit thus works like a current clamp for fast signals and like a voltage clamp for low frequency signals.
E CCGain;;When the CC-Gain is set to 1 pA/mV, a command pulse of 1 mV gives rise to 1 pA in current clamp mode. This relationship of 0.1 and 1 pA/mV holds regardless of the Gain setting. The maximal output current in Current Clamp mode is 1 / 10 / 100 nA, respectively.
E GentleSwitch;Gentle CC-Switch: ON:;I-hold will be set to inject the holding current required to keep the membrane voltage constant while switching to current clamp mode. Thus, V-mon will be identical to the original V-memb commanded under voltage clamp. In analogy, upon returning to voltage clamp, V-memb will be set according to the membrane potential under current clamp and rounded to the next 2 mV.
E VmonX100;V-mon Gain:;The measured voltage can be amplified by a factor of 10 or 100 before digitization. In the *100 setting the voltage range is +/-100 mV and the resolution 1.5µV. This is particularly useful in field potential measurements, where small voltage deflections are to be resolved.
E LastVHold;Last V-memb:;If changing from VC to CC mode, the software remembers the previously set holding potential. Last V-memb. will set this holding potential after changing modes.
E Bell;Bell:;Plays the system sound. This is useful to supply feedback when executing a macro.
E Relative;Relative Value:;This button is useful when recording macros. A change of any value will be recorded as a relative change, e.g. if you hyperpolarize the holding potential from -60 to -70 mV while recording a macro, a step of -10 mV will be recorded
E TestOverlay;Overlay:;The Test Pulse traces will be overlaid in the Oscilloscope window.
E SoundHz;Hz/MOhm;Here the settings for the sound feature can be entered. The sound is played proportional to R-membrane. The sensitivity (Hz/MOhm) and volume (in %) of the sound encoding of R-membrane can be specified there.
E SoundVol;Volume:;Here the settings for the sound feature can be entered. The sound is played proportional to R-membrane. The sensitivity (Hz/MOhm) and volume (in %) of the sound encoding of R-membrane can be specified there.
E Wait;Wait:;This control is used to pause a macro execution for the given time.
N Status;State:;Current state of the experiment.
N Time;Time:;Actual system time measured since midnight.
N Timer;Timer:;This item functions as a stopwatch or timer called TimerTime. It can be reset at any time by clicking on the Set item or by pressing Shift + T. It may be useful to keep track of the experiment (e.g., to monitor the time spent in a specific experiment mode).
N Comment;Comment:;Comment to the currently active series. This field can be edited. It will result in a modification of the text of the present series if a file was opened with write permission. This means that one has to enter a text to a series after it has been acquired. The new comment will be copied into all incoming new series until a new text is entered.
N Store;Store:;Store is used to save the data that will be acquired from now on. A dialog window opens and you have to enter the name of the new data file.
N Break;Break:;Break is used to stop series acquisition or replay. The Break flag is reset, when the next target is displayed or executed. If Break is pressed during acquisition of a sweep, this particular sweep will not be completed and the data acquired for this sweep thus far will be discarded. All previous sweeps of the series will be saved
N Stop;Stop:;This button is used similarly to Break. However, during acquisition of a sweep, this particular sweep will be completed. The Stop button can also be used in Replay mode when performing a lengthy operation.
N Next;Next:;At the end of the actually acquired sweep the series is stopped and the next protocol event will be executed.
N Wait;Wait:;This button is used to pause series execution. Its command key is Ctrl + W. Series execution is resumed by a click on Resume or by Ctrl + R. Wait will become effective after completion of the currently acquired sweep. The Wait button can also be used in Replay mode when performing a lengthy operation; e.g., it allows inspecting a particular sweep, when replaying a collection of sweeps.
N Resume;Resume:;Opposite of Wait. Press Resume to continue data acquisition or replay.
N Average;Average:;By use of this function, a PGF sequence can be repeated several times. Only the average is stored to disk. Please enter the number of averages acquired for one sweep. It is not mandatory but advised to use even numbers of averages in order to avoid possible problems with the Alternating Leak Averaging feature (see page topic:AltLeakAv).
B Go;Go:;% Runs the selected %protocol.
B Store;Store:;% Store is used to save acquired data. If you start a new %data file, a dialog window will open and the name of the file %entered.
B Break;Break:;% Break is used to immediately stop series acquisition. The %Break flag is reset when the next target is displayed or executed.
B Stop;Stop:;% This button is used similarly to Break. %However, during acquisition of a sweep, this particular sweep will %be completed.
B Next;Next:;% At the end of the %actually acquired sweep the series is stopped and the next %protocol event will be executed.
B Wait;Wait:;% This button is used to pause series execution. Its command %key is Ctrl + W. Series execution is resumed by a %click on Resume or by Ctrl + R. Wait will become %effective after completion of the currently acquired sweep. The %Wait button can also be used in Replay mode when performing a %lengthy operation; e.g., it allows inspecting a particular sweep, %when replaying a collection of sweeps.
B Resume;Resume:;% Opposite of Wait. Press Resume to continue data %acquisition.
B Load;LOAD/SAVE:;Loads or saves the pool of available protocols (*.pro file).
B Save;LOAD/SAVE:;Loads or saves the pool of available protocols (*.pro file).
B Name;;The name of the present protocol is displayed in the blue field. To edit this name, click into the field. End editing either by pressing Return or by clicking with the mouse into another area of the window.
B New;NEW:;Creates a new protocol in the pool.
B Delete;DELETE:;Removes the present protocol from the pool.
B Step;STEP:;Steps to the next line in the event list.
B ToEnd;TO END:;Runs the protocol through to its end.
B ShowEvents;Show:;Updates the event list to highlight the currently executed event during execution of a protocol.
B WriteEvents;Write:;Writes the events to the Notebook window during execution of a protocol.
B ListEvents;LIST:;Lists the present protocol in the Notebook window.
B MoveEvents;MOVE:;Moves the present protocol to another position in the protocol pool.
S Save;SAVE:;Saves the configuration file with its current name.
S SaveAs;SAVE AS...:;Saves the configuration file under a new name.
S SetWinDef;Default Windows:;resets the position of most windows. This helps to restore the windows position once one gets dragged off screen (for example after reducing the screen resolution).
S SetDef;Default Settings:;Sets all settings back to the original settings. Note that this needs a restart of the program!
S WindowScale;Window Scaling:;This feature is used to scale (change size of all controls) all windows. This may be useful when there is too little space for all necessary windows on a single monitor. The new scaling only takes effect after saving the configuration file and upon restarting the program.
S MaxPoints;Max. Sample Points:;Sets the maximum number of points that can be acquired in one trace
S MaxTraces;Max. Stim. Traces x Points:;Maximum number of stimulation traces and their respective number of points. This parameter is limited by the amount of available memory.
S MaxStim;Max. Stim. Traces x Points:;Maximum number of stimulation traces and their respective number of points. This parameter is limited by the amount of available memory.
S MinWait;Min. Wait Time:;Sets the time PATCHMASTER reserves to wait for the correct time to start when executing individual repeat loops such as acquisition of a series of sweeps or an Repeat loop in the Protocol Editor.
S MacroTimeout;Macros Timeout:;: a timeout for the execution of macros can be set. After that time the execution of a macro is aborted.
S BatchComm;Batch Communication:;The "Batch Communication" with other applications (see chapter ch:controllingPM) can be disabled or enabled.
S Username;User name:;a user name that is entered here will be stored with the raw data.
S Amplifier;Amplifier:;From this list one can select the patch clamp amplifier used.
S ADBoard;Digitizer Selection:;Here one can select the appropriate AD/DA-converter, if it is not automatically assigned via the amplifier selection.
S VCScale;VC: Stim. Scale:;Scaling factor of the stimulus signal in Voltage Clamp mode. The voltage amplitude that is set in the pgf sequence is divided by this factor before output to the DA-channel.
S CCScale;CC: Stim. Scale:;Scaling factor of the stimulus signal in Current Clamp mode. The current amplitude that is set in the pgf sequence is multiplied by this factor before to convert it to a command voltage output at the DA-channel. This factor has to be adjusted with respect to the Ext. Stim Input Scaling in Current Clamp mode of the controlled amplifier to guarantee that the current amplitudes specified in the pgf sequence are identically to those that the amplifier applies to the cell membrane.
S VoltInScale;Voltage In Scale:;Scaling factor of the voltage input signal. For all HEKA amplifiers this factor is fixed at 0.1.
S CurrentIn;Current In:;Assigns the input AD-channel for the current trace. This input channel is scaled by the Current Gain
S VoltageIn;Voltage In:;Assigns the input AD for the voltage trace.
S VmembOut;V-membrane Out:;Assigns the output DA-channel of the stimulus.
S TestTrig;Test Trigger Out:;Assigns the output channel of the trigger that is set at the beginning of each test pulse. The Test Trigger Out should be turned off if the digital oscilloscope of PATCHMASTER (Oscilloscope window) is used. Turn it on if you would like to trigger an external oscilloscope.
S SerialOut;;This control is used to open and set up the serial port communication mode. If a so-called Serial Communication has been established between the computer and another device, PATCHMASTER can send strings over a serial port, but it will not receive instructions.
S LockIn;;Here the Lock-In amplifier of PATCHMASTER can be activated.
S Photo;;This will activate stimulation of a monochromator and acquisition via a photo-multiplier or photo diode.
S AutoFilter;Auto Filter:;The Pulse Generator window (see Chapter Pulse Generator) provides the option to automatically set a hardware filter according to the chosen sample interval. Auto Filter enables and disables this automatic filter setting, which is only supported for the EPC 10 and EPC 9 with its built-in hardware filters.
S HomePath;Home Path:;Sets the path to the folder LookupTables, to all window dialog files (*.dia) as well as to the key assignment file PATCHMASTER.key. It is also the path where the default files DefPgf_v9.pgf, DefAnal.onl and DefProt.pro are searched automatically as long as no configuration file was saved.
S DataPath;Data File:;Sets the path to the data file (file extension *.dat)..
S ProtFile;Protocol File:;Sets the path to the protocol files (file extension *.pro)..
S PgfFile;PGF Pool File:;Sets the path to the pulse generator file (file extension *.pgf).
S MacroFile;Macro File:;Sets the path to the macro files (file extension *.mac).
S OnlineFile;Online File:;Sets the path to the Online Analysis file (file extension *.onl).
S SolFile;Solution Base:;Sets the path to the solution data base files (file extension *.sol).
S BatchPath;Batch Path:;Path for batch file control protocols, e.g., E9BatchIn and E9BatchOut
S TempPath;Temp. Path:;Optionally a path different from the home path can be specified for storing temporary data.
S IGainTable;I-gain Lookup:;Path to the Current Gain lookup table. Another lookup table for the Current gain (see section subsec:Config_IO_Parameters I/O Parameters, page subsec:Config_IO_Parameters). Only necessary for telegraphing amplifiers.
S SaveSet;Save Settings File:;The Configuration settings will be saved automatically on exit, thus overwriting the previous settings.
S Verify;Verify Quit:;When this option is enabled, PATCHMASTER will ask to save modified settings files (*.pgf, *.pro, *.onl) when you quit the program. When it is not enabled, the Configuration settings will not be saved on quit!
S AskData;Ask for Data File:;When this option is enabled, PATCHMASTER will ask for a data file upon startup.
S SaveBreak;Save after Break:;When this option is enabled, PATCHMASTER will save the data in a sweep that have been acquired when the acquisition was terminated via the Break button. With this option disabled the entire sweep will be discarded if during its acquisition the Break button was pressed.
S Bundle;Make Bundle Files:;When this option is selected, all files generated for a data set (raw data, "pgf", "pul", etc.) are combined into one file.
S SolBase;Solution Base:;sec:configuration_solution When this option is selected, you are prompted to load an existing solution data base file or create a new file.
S NameDescr;Auto Filename:;Allows an automatic filename generation. The filenames can be generated out of a set of special components.
S StartMacro;Startup Macro:;Allows entering the name of a macro that is executed after the end of program initialization (i.e. after the configuration file is loaded, the amplifier initialized and the other settings activated). E.g., allows for additional settings like the immediate activation of the second amplifier of an EPC Double at startup.
S ExpNumber;Experiment Number:;This number can be used to identify experiments. It is automatically incremented when a new Experiment is generated with File -> New Experiment.
S MaxFile;Max. File Size:;This feature will not limit the size of any file written to disk, but can be used as a reminder so that no files are created that e.g., do not fit onto a zip drive. A warning is written to the Notebook if the size of the raw data file exceeds this value. Entering a very large number will - in practice - suppress any warnings.
S Fonts;Fonts / Button Colors / Line Colors:;Colors and text fonts for the program layout can be selected here. These are global settings for all window dialogs and they are installed upon restart of PATCHMASTER. The colors and fonts are stored in the configuration file (*.set), i.e., they are independent of the dialog files
S ButtonCol;Fonts / Button Colors / Line Colors:;Colors and text fonts for the program layout can be selected here. These are global settings for all window dialogs and they are installed upon restart of PATCHMASTER.The colors and fonts are stored in the configuration file (*.set), i.e., they are independent of the dialog files
S LineCol;Fonts / Button Colors / Line Colors:;Colors and text fonts for the program layout can be selected here. These are global settings for all window dialogs and they are installed upon restart of PATCHMASTER.The colors and fonts are stored in the configuration file (*.set), i.e., they are independent of the dialog files
S NoteDigs;Notebook:;(range 6-24) Numbers of digits for data display in the Notebook window.
S TimeDigs;Time:;(range 8-11) Numbers of digits for the time and timer function in the Oscilloscope window. The maximal number is 11, this results in "hh:mm:ss:ms" (milliseconds).
S XGridDigs;Grid Digits X|Y:;(range 6-10) Number of digits to be shown in grid annotation of the Oscilloscope and the stimulation template preview of the Pulse Generator.
S YGridDigs;Grid Digits X|Y:;(range 6-10) Number of digits to be shown in grid annotation of the Oscilloscope and the stimulation template preview of the Pulse Generator.
S GraphdX;Graph 3D dX|dY:;(range 0.001 -- 1.000) Allows to specify the horizontal and vertical offset of subsequent sweeps to be displayed in the 3D graphing mode. You can also enter 0 for one or both. Note that the 3D-Graph feature has to be On for this option to take effect
S GraphdY;Graph 3D dX|dY:;(range 0.001 -- 1.000) Allows to specify the horizontal and vertical offset of subsequent sweeps to be displayed in the 3D graphing mode. You can also enter 0 for one or both. Note that the 3D-Graph feature has to be On for this option to take effect
S Round;Round:;Round to mV will round voltages specified in the segments of a stimulation template to mV. Round to DAC: will round voltages specified in the segments of a stimulation template to the minimal digitization unit. Use this option when a higher precision than mV is needed for segment voltages.
S List;List:;Pressing this button writes all scaling and offset values for the I/O parameter in the notebook window. Use this feature for getting a quick overview on the scaling of all your AD and DA channels.
S ScaleScale;Scale:;If a parameter is read via an AD-channel, a scaling feature allows the acquired voltage to be converted into the desired variable.  You can choose between a Scaling Factor and a Lookup table.
S IGainScale;Table:;Config_ButtonTable.png If only a lookup table can be used for conversion of the sampled analog voltage to the parameter value, this button appears. This is the case, e.g. for conversion of the voltages from the telegraphing outputs of connected amplifiers to gain or bandwidth settings.
S IGainShow;Current Gain:;Gain of the current monitor input. This parameter scales the current signal sampled from the Current Monitor channel specified in the Output and Input Channel Settings. When using a telegraphing amplifier the AD-channel that samples the gain telegraph voltage has to be specified and the gain lookup table has to be chosen.
S AdcScale;;AD-Channel: If data traces from AD-channels are acquired in a pgf sequence, the voltages can be scaled to convert them to appropriate units. For scaling you can use a fixed value or lookup tables.  In case you have an EPC 8 or EVA 8 amplifier connected as second amplifier then use EPC 8 Gain to scale the AD-channel that samples the Current Monitor signal of the EPC 8. The corresponding EPC 8 has to be connected via the parallel digital I/O interface and the amplifier set to local mode.
S DacScale;;DA-Channel: Free DA-output channels that can be used for stimulation during a pgf sequence can be scaled with a factor entered in the Value field.
S User1Name;User Parameter 1, 2 :;There are two parameter fields reserved for user-specific assignments. These fields act like other parameter fields with the addition that parameter name and unit can be specified (e.g., "pH" and "U"). Name and unit are also stored in the output *.pul file on the level of each sweep, so they can be changed during an experiment, if required.
S User2Name;User Parameter 1, 2 :;There are two parameter fields reserved for user-specific assignments. These fields act like other parameter fields with the addition that parameter name and unit can be specified (e.g., "pH" and "U"). Name and unit are also stored in the output *.pul file on the level of each sweep, so they can be changed during an experiment, if required.
S BandwidthShow;Bandwidth:;Recording bandwidth.
S PLPhaseShow;PL-Phase:;Phase angle of a lock-in amplifier.
S TemperatureShow;Temperature:;Temperature (from a recording device).
S CSlowShow;C-slow:;Membrane capacitance.
S RseriesShow;R-series:;Series resistance.
S LeakCompShow;Leak Comp:;Leak conductance.
S RsValueShow;Rs-value:;Series resistance compensation.
S CellPotShow;Cell Potential:;Cell potential.
S PipPressShow;Pipette Pressure:;Pressure (from an external device).
S PipResShow;Pipette Resist.:;Value of R-membrane after pressing W (this will write the value of R-membrane into Pipette Resistance.
S SealResShow;Seal Resistance:;Value of R-membrane.
S NoiseShow;RMS Noise:;Value of the RMS noise.
S SolMode;Show Solutions:;Config_ShowSolutions.png From a drop list you can choose if External and Internal solution numbers will be displayed and can be edited in the I/O Control window. In case you run an EPC with multiple headstages the option Show Amplifier Solutions allows to specify different internal solutions for each amplifier.
S OnlineSwps;Online Sweeps | Results:;(sweeps min. 256, result min. 8) The Online Analysis needs to allocate memory for storage of its results. The online result buffer is a 2-dimensional buffer: number of results (= number of online functions) x number of times the online analysis is executed (= number of entries on the x-axis of a full scale online analysis graph).
S OnlineResults;Online Sweeps | Results:;(sweeps min. 256, result min. 8)  The Online Analysis needs to allocate memory for storage of its results. The online result buffer is a 2-dimensional buffer: number of results (= number of online functions) x number of times the online analysis is executed (= number of entries on the x-axis of a full scale online analysis graph).
S OnlineMode;Online Mode:;Handling of Online Sweeps | Results:   - "Fixed": The number of Online Sweeps | Results given above is limited to that number. - "Extend": The number of Online Sweeps | Results will be automatically extended, if more data are acquired. - "Wrap": If the number of Online Sweeps | Results is higher than set above, the new data will be saved from the beginning of the buffer again and thus overwrite the older data.
S FreezeZero;Freeze Zero Line:;This checkbox determines the behavior of the display scaling in the Oscilloscope Window. When Freeze Zeroline is selected, the oscilloscope behaves like an oscilloscope, i.e. when the display gain is changed the zero line stays at its position. When deselected, display scaling is performed with reference to the center of the display. I.e., when Y-offs is not zero, the zero line may alter its position on the screen.
S TestFilt;Test Filter Factor:;The EPC 10 filter is set according to the sample interval of the built-in test pulse using this Test Filter Factor. Of course, this only is active when is Auto Filter is on. The Test Filter Factor can be turned off by setting the value to zero.
A Type;Function Type Button:;Here the function type of the presently activated analysis function is displayed. Click on it to open the Function type window.
A NewFunction;NEW:;Creates a new analysis function. You can also create a function by clicking on an empty function button.
A DelFunction;DELETE:;Deletes the selected analysis function.
A MoveFunction;MOVE:;Changes the position of a particular analysis function within the scroll bar.
A XOffs;X-, Y-Segment Offsets:;The X- and Y-Segment-Offsets are set relative to the Relevant X{Pulse Generator window.
A YOffs;X-, Y-Segment Offsets:;The X- and Y-Segment-Offsets are set relative to the Relevant X- and Y-Segments as defined in the Pulse Generator window.
A Cursor;Cursor Bounds:;Within the relevant segment, a left bound and a right bound (in %) determine the actual time period for analysis. Pressing the button lets popup the cursors in the Oscilloscope window.
A Trace;Trace 1.."Max. Shown Traces" :;Select which trace shall be analyzed using the analysis functions.
A TargetVal;not stored in Value / Store to Value:;Analysis results can be stored in one of the values 1.."Max. Shown Values". They can then be retrieved and used as a Constant Math function. In addition, they can be used in the Protocol Editor (see section Set Value, page subsec:_PE_SetValue). The Values 1.."Max. Shown Values" are used to exchange information between different Analysis Methods and between the Online Analysis and the Protocol Editor.
A Notebook;Notebook:;If selected, the analysis result of this function will be written into the Notebook window.
A DoFit;Fit:;This checkbox only appears for the analysis functions Peak Amplitude, Extremum, Minimum, Maximum, and Time to Peak. If selected, the peak value is determined by a polynomial fit around the first numeric estimate of the peak. With this function, the influence of noise can be reduced in the determination of peak amplitudes.
A Gr1WinNr;Graph Display Mode:;A graph can either be turned on or off.
A Gr2WinNr;Graph Display Mode:;A graph can either be turned on or off.
A Gr3WinNr;Graph Display Mode:;A graph can either be be turned on or off.
A Print;Print:;Prints the online analysis results as displayed.
A GrCopy;Copy:;Copies settings from another graph into the selected one. Allows to automatically copy the settings of a graph into the selected graph, rather than having to enter each entry manually.
A Redraw;Redraw:;Redraws the online display, e.g. after changing the axis scaling.
A Overlay;Overlay:;If you mark this option, the graph plot in the Analysis window will not be cleared when performing a new analysis.
A Wrap;Wrap:;Selecting Wrap leads to a wrapping of the data at the end of the display, e.g. the display will start again at the left edge. The Wrap option is limited to Fixed Scaling of the X-axis and Y-axis and the X-axis mode has to be linear.
A OvrlSwp;OvrlSwp:;Overlay Sweep function overlays all sweeps within a series.
A XMin;Min:;Minimum of axis range.
A XMax;Max:;Maximum of axis range.
A XScale;Scale:;There are several possible scaling options available once you click on the Scale window.
A SharedX;Share X-axis:;If Share X-axis is checked, then the same x axis parameters (e.g. "Min", "Max", "Scale") are used for all graphs
A ScMore;Modify Axis:;Clicking on this button opens the Scale Properties window.
A ENr1;1...4:;Activate up to four entries from this list by checking the box.
A EnX0;Axis:;Now choose from the list of available analysis result variables for the X- and the Y-axis.
A Load;LOAD:;Loads a pool of Analysis Methods.
A Merge;MERGE:;Adds Analysis Methods from an Online Analysis file (*.onl) to the already loaded pool of methods.
A Save;SAVE:;Saves the actual pool of Analysis Methods.
A Name;Analysis Method Name:;Text field in which the name of the current Analysis Methods can be edited
A New;NEW:;Creates a new Analysis Method.
A Delete;DELETE:;Deletes the active Analysis Method.
A Move;MOVE:;Changes the positioning of a particular Analysis Method within the scroll bar.
