Jon Newman
2010 12 21
Effect of CNQX on Decodability
-------------------------------

In this experiment, I aim to measure the effects of increasing concentrations of the competitive AMPA anatognist, CNQX, on the ability of the culture to respond to stimuli of various rates. Detailed notes on how I came up with the protocol can be found in NB.P.005 around pages 30 to 40.

I am using my standard Poisson Stimulation, Uniform over electrodes paradigm with the following parameters:
-Stim rates = 5 15 and 50 Hz
-Epochs = 10 for each rate, each lasting 60 seconds

Spike Detection
-Spike filter is 1st order with [500 5000] Hz passband
-Spike detection: 8*RMS noise, Fixed
-SALPA is being used with 2.5 ms blanking period following stimuli.


Each protocol starts with a 30 minute spontaneous recording to let the drugs take effect.

Bad channels = [35 22 27] -> more artifacts than spikes during stim protocols. Should be removed from analysis.

Recordings
-----------------

1. [CNQX] = 0uM

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_0um-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_0um-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_0um-t2


2. [CNQX] = 1uM

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_01um-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_01um-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_01um-t2

3. [CNQX] = 5uM

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_05um-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_05um-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_05um-t2

4. [CNQX] = 10uM

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_10um-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_10um-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_10um-t2

	- Spontaneous activity is non-existant at this point. Seems a bit extreme.
	- There are certainly evoked spikes but the percentage that are artifacts is dialing up, presumably due to a decrease in spike rate.

5. [CNQX] = 20uM

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_20um-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_20um-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_20um-t2

	- Spontaneous activity is really non-existant at this point, not a single spike in a half hour
	- Getting about 1 spike per stim at this point. Vast majority of detected spikes are junk. 
	- I'm calling it at this concentration, next recording will be wash

6. Wash 1 - media removed 3x and replaced 3x

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_wash01-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_wash01-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_wash01-t2

	- Spontaneous activity is returning in the form of bursting. My goodness bursting is there anything that will desync these cultures for any period of time?
	- Spontaneous activity looks good. Files sizes are approaching those of the 0 concentration scenario.

7. Wash 2 - media removed 3x and replaced 3x

	- 30 minutes spont. activity
		> fid: 2010-12-21_16259-optimus_wash02-pt
	- Stim protocol trial 1
		> fid: 2010-12-21_16259-optimus_wash02-t1
	- Stim protocol trial 2
		> fid: 2010-12-21_16259-optimus_wash02-t2