This is the data set from D. A. Wagenaar, J. Pine, and S. M. Potter, 2006, "An extremely rich repertoire of bursting patterns during the development of cortical cultures", BMC Neuroscience, 7:11. Downloading from this site is subject to the condition that you acknowledge our work in any publications that use our data, by citing this article.
Results: Plating density had a profound effect on development. While the aggregate spike detection rate scaled linearly with density, as expected from the number of cells in proximity to electrodes, dense cultures started to exhibit bursting behavior earlier in development than sparser cultures. Analysis of responses to electrical stimulation suggests that axonal outgrowth likewise occurred faster in dense cultures. After two weeks, the network activity was dominated by population bursts in most cultures. In contrast to previous reports, development continued with changing burst patterns throughout the observation period. Burst patterns were extremely varied, with inter-burst intervals between 1 and 300 s, different amounts of temporal clustering of bursts, and different firing rate profiles during bursts. During certain stages of development bursts were organized into tight clusters with highly conserved internal structure.
Conclusions: Dissociated cultures of cortical cells exhibited a much richer repertoire of activity patterns than previously suspected. Except for the very sparsest cultures, all cultures exhibited globally synchronized bursts, but bursting patterns changed over the course of development, and varied considerably between preparations. This emphasizes the importance of using multiple preparations___not just multiple cultures from one preparation___in any study involving neuronal cultures. These results are based on 963 half-hour-long recordings. To encourage further investigation of the rich range of behaviors exhibited by cortical cells in vitro, we are making the data available to other researchers, together with Matlab code to facilitate access.
Synaptic Upscaling and Optoclamp Data